高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (5): 821.

• 研究快报 • 上一篇    下一篇

d(GGTATACC)2与乙二胺四乙酸在溶液中的相互作用

阎江丽1,2, 毛希安1, 沈联芳1, 张友杰2   

  1. 1. 中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室, 武汉, 430071;
    2. 华中师范大学分析测试中心, 武汉, 430079
  • 收稿日期:1997-12-08 出版日期:1998-05-24 发布日期:1998-05-24
  • 通讯作者: 毛希安
  • 作者简介:阎江丽,女,24岁,博士研究生,副教授.
  • 基金资助:

    国家自然科学基金;武汉市晨光计划资助

Interaction of d(GGTATACC)2 and EDTA in Solution

YAN Jiang-Li1,2, MAO Xi-An1, SHEN Lian-Fang1, ZHANG You-Jie2   

  1. 1. State Key Laboratory of Magnetic Resonance and Atomicand Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071;
    2. Center for Analysis and Testing, Central China Normal University, Wuhan, 430079
  • Received:1997-12-08 Online:1998-05-24 Published:1998-05-24

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关键词: NOESY, d(GGTATACC)2, EDTA, 分子间相互作用

Abstract: When three dimensional conformations of nucleic acid are studied with NMR methods, EDTA(ethylene diamine tetra-acetic acid) or its sodium salt has to be added in the solution in order to protect the nucleic acids from contamination by some metal ions such as Mg2+ . In this paper, the interaction of d(GGTATACC)2 and EDTAhas been observed in D2O solution containing 0.05mmol/L DNA and about 5 mmol/L EDTA. Four pairs of NOE cross peaks connecting the acetic proton(δ 3.64) of EDTA with the protons G18(δ 7.81), G11′(δ 5.65), G13′(δ 4.13) and G12′(δ 2.54) of d(GGTATACC)2 observed, suggesting that EDTA may form complex with d(GGTATACC)2 possibly through hydrogen bond interactions between amino protons in DNA (H2 of G1 and H4 of C8) and carbonyl oxygen atoms of EDTA. The interaction of d(GGTATACC)2 and EDTA may account for NOEs between two DNA oligomers, G11′(5.65) and C86(7.47), G11′(5.65) and C82′/C82″(2.18).

Key words: NOESY, d(GGTATACC)2, EDTA, Intermolecular interactions

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