高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (12): 1975.

• 论文 • 上一篇    下一篇

曼氏血吸虫谷胱甘肽S-转移酶Sm26/2的抗原肽研究

许家喜1, 蔡孟深2   

  1. 1. 北京大学化学与分子工程学院, 北京, 100871;
    2. 北京医科大学药学院, 北京, 100083
  • 收稿日期:1997-12-01 出版日期:1998-12-24 发布日期:1998-12-24
  • 通讯作者: 许家喜,男,34岁,博士,副教授.
  • 作者简介:许家喜,男,34岁,博士,副教授.
  • 基金资助:

    国家自然科学基金(批准号:29772002)资助课题.

Studies on Antigenic Peptides of Glutathione S-Transferase Sm26/2 of Schistosoma Mansoni

XU Jia-Xi1, CAI Meng-Shen2   

  1. 1. College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871;
    2. School of Phamarceutical Sciences, Beijing Medical University, Beijing, 100083
  • Received:1997-12-01 Online:1998-12-24 Published:1998-12-24

PDF (PC)

被引次数

3

摘要： 在计算机辅助下,应用Goldkey和PC-Gene程序,根据新报道的曼氏血吸虫谷胱甘肽S-转移酶Sm26/2的氨基酸序列,进行亲水性、柔韧性、可接近性、电荷分布和二级结构分析,预测出6个抗原肽,并用固相法进行了合成.经Dot-ELISA法测定,其中的2个对抗日本血吸虫免疫球蛋白多克隆抗体(抗-Sj-IgG-PcAb)显示出较好的抗原性,1个对抗血吸虫表膜单克隆抗体(A6McAb)显示出较好的抗原性,可作为抗血吸虫病合成多肽疫苗的候选肽段.

关键词: 曼氏血吸虫, 谷胱甘肽S-转移酶, 抗原肽, 多肽疫苗

Abstract: According to the hydrophilicity, flexibility, accessibility, charge distribution and β-turn in the secondary structure by the determination of the primary structure, six antigenic peptides of 26000 glutathione S-transferase, Sm26/2, of schistosoma mansoni have been predicted by using programs Goldkey and PC-Gene with the aid of computer. They were synthesized by using solid phase method. Two of them showed a good antigenicity by Dot ELISAmethod with anti schistosoma japonicum-immunoglobulin polyclonal antibody, anti Sj-IgG PcAb. One of them also showed good antigenicity with the anti-schistosoma surface membrane monoclonal antibody, A6McAb. They would be as candidate peptides of synthetic peptide vaccine against schistosomiasis.

Key words: Schistosoma mansoni, Glutathione S-transferase, Antigenic peptide, Peptide vaccine

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