高等学校化学学报

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主流卷烟烟气中气相自由基测定方法的改进

周骏1,3, 吴可2, 孙岳3 , 丛建波2, 王长振2, 常翔3, 先宏2, 朱永法1   

    1. 清华大学化学系, 北京 100084;
    2. 军事医学科学院放射与辐射医学研究所, 北京 100850;
    3. 北京卷烟厂技术中心, 北京 100024
  • 收稿日期:2007-04-29 修回日期:1900-01-01 出版日期:2007-10-10 发布日期:2007-10-10
  • 通讯作者: 朱永法

Development of a Quantitative Method for Analyzing Gas-phase Free Radicals in Mainstream Cigarette Smoke

ZHOU Jun1,3, WU Ke2, SUN Yue3, CONG Jian-Bo2, WANG Chang-Zhen2, CHANG Xiang3, XIAN Hong2, ZHU Yong-Fa1*   

    1. Department of Chemistry, Tsinghua University, Beijing 100084, China;
    2. Beijing Institute of Radiation Medicine, Beijing 100850, China;
    3. Technology Center, Beijing Cigarette Factory, Beijing 100024, China
  • Received:2007-04-29 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10
  • Contact: ZHU Yong-Fa

摘要: 按照ISO 4387标准进行吸烟, 通过自行研制的捕集装置, 以2-苯叔丁基硝酮(PBN)的四氯化碳溶液捕集气相自由基. 将捕集到气相自由基的溶液先用液氮冷冻, 再定时解冻以抑制其衰减, 并有效地去除氧气对气相自由基定量分析的干扰, 获得理想的测定重复性. 用电子顺磁共振波谱仪(EPR)检测气相自由基, 以2,2,6,6-四甲基哌啶-1-氧(TEMPO)作为标准样品, 以碳酸钙稀释的煤粉作为校正样品, 定量计算气相自由基. 测定方法有较好的重复性和较高的灵敏度, 可以满足日常卷烟烟气气相自由基的定量分析的需要.

关键词: 主流卷烟烟气, 气相自由基, 定量分析, 电子顺磁共振

Abstract: An improved method was developed for the determination of the gas-phase free radicals in mainstream cigarette smoke. A trap device for the gas-phase free radicals was developed, which could be easily attached to and removed from a commercial linear smoking machine. N-t-Butyl-α-phenylnitrone(PBN) was used to trap the gas-phase free radicals and the formed adduct was detected with EPR technique. The adduct was immediately frozen in liquid nitrogen for degassing and stabilization of the adduct. 2,2,6,6-Tetramethyl-piperidinyl-l-oxy(TEMPO) and strong pitch was used as calibration samples to quantitatively analyze the gas-phase free radicals. The sampling, conditioning and selection of cigarettes in the test were accordant with ISO 4387. It was proved that the established method was highly sensitive, reproducible, practicable and accurate for the detection of the gas-phase free radicals in routine tests.

Key words: Main-stream cigarette smoke, Gas-phase free radicals, Quantitative analysis, Electron Paramagnetic Resonance(EPR)

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