高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

哮喘大鼠淋巴细胞蛋白质组的分析

李善玉1,2, 范佳1, 席景会1,3, 张海玉2, 杜宏伟2, 周欣1, 王旭1, 宁波1, 刘兰英4, 郝东云1   

    1. 吉林大学分子酶学工程教育部重点实验室,
    2. 吉林大学第一医院, 长春 130021;
    3. 吉林大学植物科学学院, 长春 130062;
    4. 吉林大学生命科学院, 长春 130021
  • 收稿日期:2006-12-01 修回日期:1900-01-01 出版日期:2007-09-10 发布日期:2007-09-10
  • 通讯作者: 郝东云

Spleen Lymphocyte Proteome Analysis in Bronchial Asthma of Wistar Rats

LI Shan-Yu1,2, FAN Jia1, XI Jing-Hui1,3, ZHANG Hai-Yu2, DU Hong-Wei2, ZHOU Xin1, WANG Xu1, NING Bo1, LIU Lan-Ying4, HAO Dong-Yun1*   

    1. Key Laboratory for Molecular Enzymology & Engineering of Ministry of Education,
    2. The First Clinical Hospital, Norman Bethune Medical College, Jilin University, Changchun 130021, China;
    3. College of Plant Science, Jilin University, Changchun 130062, China;
    4. College of Life Science, Jilin University, Changchun 130021, China
  • Received:2006-12-01 Revised:1900-01-01 Online:2007-09-10 Published:2007-09-10
  • Contact: HAO Dong-Yun

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被引次数

7

摘要： 通过建立卵蛋白(Ovalbumin, OVA) 致敏的Wistar大鼠哮喘动物模型, 利用二维凝胶电泳及MALDI-TOF-MS分析技术, 对哮喘大鼠与正常大鼠脾脏淋巴细胞差异表达蛋白质进行鉴定和分析. 结果显示, 在哮喘组中呈现高表达的蛋白质, 包括胶原蛋白调控蛋白质(hnRNP)以及细胞结构蛋白(Fibrinogen). 而与代谢相关的蛋白质(CoQ10)和线粒体内膜蛋白, 在哮喘组中呈低表达. 研究结果表明, 这些蛋白质与哮喘气道慢性炎症、气道高反应性和气道的重构有关.

关键词: 支气管哮喘, 淋巴细胞, 蛋白质组, 二维电泳/质谱

Abstract: To study the molecular mechanisms of asthma, the bronchial asthma model was established with OVA-sensitized Wistar rats. The differentially expressed spleen lymphocyte proteomes between asthmatic rats and normal rats were profiled and the proteins were identified via two-dimensional electrophoresis and MALDI-TOF-MS. The results reveal that the expression of some protein genes was up-regulated in asthmatic rats in comparison with the normal rats. These includes hnRNPK, involving the transcriptional regulation of collagen proteins, and cell structural protein Fibrinogen. However, the energetic metabolism-relating protein such as CoQ10 and mitochondrial inner membrane protein were exhibited to be down-regulated. Further analysis using bioinformatic tools suggests that the above-mentioned proteins are likely important components involving the airway chronic inflammation, airway hyper-responsiveness and airway remodeling of asthmatic rats.

Key words: Bronchial asthma, Lymphocyte, Differential proteome, 2-DE/MS

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