高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (3): 371.

• 研究论文 • 上一篇    下一篇

非水介质毛细管电泳电化学检测日夜百服宁中的有效成分

郑一宁1, 谢天尧1, 莫金垣1, 韦寿莲2   

  1. 1. 中山大学化学系, 广州 510275;
    2. 广西民族学院化学化工系, 南宁 530006
  • 收稿日期:2001-03-15 出版日期:2002-03-24 发布日期:2002-03-24
  • 通讯作者: 莫金垣(1934年出生),男,教授,博士生导师,主要从事电分析和化学计量学研究.E-mail:cesmjy@zsu.edu.cn E-mail:cesmjy@zsu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:9675033);广东省自然科学基金(批准号:001237)资助

Determination of Effective Components in Riyebaifuning Tablet by Nonaqueous Capillary Electrophoresis with Electrochemical Detection

ZHENG Yi-Ning1, XIE Tian-Yao1, MO Jin-Yuan1, WEI Shou-Lian2   

  1. 1. Department of Chemistry, Zhongshan University, Guangzhou 510275, China;
    2. Department of Chemistry and Chemical Engineering, Guangxi Universityf or Nationalities, Nanning 530006, China
  • Received:2001-03-15 Online:2002-03-24 Published:2002-03-24

PDF (PC)

被引次数

20

摘要： 用非水毛细管电泳电化学检测法分离检测了日夜百服宁中的有效成分,研究了电极电位、不同浓度的甲酰胺(FA)、电解液浓度和酸度、电泳电压及进样时间对电泳分离的影响,得到了较为优化的测定条件.实验结果表明,在25mmol/LTris-25mmol/LH3BO3(表观pH=8.5)运行介质中,日夜百服宁中的4种有效成分即扑热息痛(AP)、盐酸伪麻黄碱(PH)、氢溴酸右美沙芬(DM)和扑尔敏(CM)在12min内完全分离.检测电位为+0.9V(vs.SCE).线性范围分别为AP 0.5～200mg/L;PH 0.8～300mg/L;DM 2.5～350mg/L;CM 0.5～330mg/L;检测限分别为AP 0.1mg/L;PH0.55mg/L;DM 1mg/L;CM 0.2mg/L.

关键词: 非水介质高效毛细管电泳, 电化学检测, 有效成分, 日夜百服宁

Abstract: In formamide(FA) medium, the separation and determination of the effective components in Riyebaifuning tablet by nonaqueous capillary electrophoresis with electrochemical detection in the presence of TrisH3BO3 as the background electrolyte were reported. The effect of potential of working electrode, different concentrations of FA, pH, the concentrations of background electrolyte, operation voltage and injection time on capillary electrophoresis were discussed. At the detection potential of 0.9 V(vs. SCE), acetaminophen(AP), pseudoephedrine hydrochloride(PH), dextromethorphan hydrobromide(DM), chlorphenamine maleate(CM) were separated within 12 min by using 25mmol Tris-25 mmol H3BO3(pH=8.5) as a carrier electrolyte at a positive voltage of 27 kV. There are an excellent linearity between peak current and concentration of analytes in the concentration range of AP 0.5—200 mg/L; PH0.8—300 mg/L; DM 2.5-350 mg/L; CM 0.5-330 mg/L. The detection limits(S/N=3) are AP 0.1 mg/L; PH 0.55mg/L; DM 1 mg/L; CM 0.2 mg/L.

Key words: Nonaqueous capillary electrophoresis, Electrochemical detector, Effective components, Riyebaifuning

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