现场紫外光谱及圆二色谱研究电化学还原反应诱导微过氧化物酶-11构象的转变

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (2): 321.

现场紫外光谱及圆二色谱研究电化学还原反应诱导微过氧化物酶-11构象的转变

姜秀娥, 张哲泠, 黄卫民, 刘小强, 汪尔康, 董绍俊

中国科学院长春应用化学研究所, 电分析化学国家重点试验室, 长春 130022

收稿日期:2004-02-27 出版日期:2005-02-10 发布日期:2005-02-10
通讯作者: 董绍俊(1932年出生),女,研究员,博士生导师,第三世界科学院院士,从事电分析化学、生物电化学和光谱电化学等方面的研究.E-mail:dongsj@ciac.jl.cn E-mail:dongsj@ciac.jl.cn
基金资助:
国家自然科学基金(批准号:20275036,20275037)资助

In-situ UV and CD Spectroelectrochemistry Investigation of the Electrochemical Reduction Reaction Inducing Conformational Transition of Microperoxidase-11

JIANG Xiu-E, ZHANG Zhe-Ling, HUANG Wei-Min, LIU Xiao-Qiang, WANG Er-Kang, DONG Shao-Jun

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received:2004-02-27 Online:2005-02-10 Published:2005-02-10

摘要/Abstract

摘要： 采用现场紫外光谱及圆二色谱电化学方法研究了微过氧化物酶-11的电化学还原过程.同时应用奇异值分解最小二乘法和双对数法对所得光谱数据进行处理.研究发现,电化学还原过程诱导微过氧化物酶-11的构象由无规卷曲向α螺旋转变,这为进一步理解生物电子传递过程与生物分子构象转变机理提供了基础信息.

关键词: 微过氧化物酶-11, 光谱电化学, 电子传递, 构象变化

Abstract: Electrochemical reduction process of microperoxidase-11 was investigated by in-situ UV-Vis and CD spectroelectrochemistry methods with a long optical path thin layer cell. The spectra obtained were analyzed by singular value decomposition least square and double logarithmic analysis methods. The results showed that the electrochemical reduction induced the conformational transition of MP-11 from random coil to α-helix and provided an important information for understanding biological electron transfer mechanism coupled with conformational transitions.

Key words: Microperoxidase-11, Spectroelectrochemistry, Electron transfer, Conformation change

中图分类号:

O657.1

TrendMD:

姜秀娥, 张哲泠, 黄卫民, 刘小强, 汪尔康, 董绍俊. 现场紫外光谱及圆二色谱研究电化学还原反应诱导微过氧化物酶-11构象的转变. 高等学校化学学报, 2005, 26(2): 321.

JIANG Xiu-E, ZHANG Zhe-Ling, HUANG Wei-Min, LIU Xiao-Qiang, WANG Er-Kang, DONG Shao-Jun. In-situ UV and CD Spectroelectrochemistry Investigation of the Electrochemical Reduction Reaction Inducing Conformational Transition of Microperoxidase-11. Chem. J. Chinese Universities, 2005, 26(2): 321.

参考文献

[1] Kasmi A.E.,Wallace J.M.,Bowden E.F.et al..J.Am.Chem.Soc.[J],1998,120:225—226 [2] WANG Quan-Lin(王全林),YANG Bao-Jun(杨宝军),LU Gong-Xuan(吕功煊).Chem.J.Chinese Universities(高等学校化学学报)[J],2003,24(9):1561—1566 [3] ZHANG Yu-Zhong(张玉中),ZHAO Hong(赵红),YUAN Zhuo-Bin(袁倬斌).Chem.J.Chinese Universities(高等学校化学学报)[J],2002,23(3):391—393 [4] Hildebrandt P.,Stockburger M..Biochem.[J],1989,28:6710—6721 [5] ZHENG Jun-Wei(郑军伟),GU Ren-Ao(顾仁敖),LU Tian-Hong(陆天虹).Chem.J.Chinese Universities(高等学校化学学报)[J],2002,23(1):42—45 [6] Wu L.L.,Huang H.G.,Li J.X.et al..Electrochim.Acta[J],2000,45:2877—2881 [7] Closs G.L.,Miller J.R..Science[J],1988,240:440—446 [8] Gray H.B.,Ellis W.R..Bioinorganic Chemistry[M],Mill Valley,CA:University Science Books,1994:315—363 [9] Ochiai E.J..Chem.Education[J],1997,74:600—604 [10] Hildebrandt P.,Vanhecke F.,Buse G.et al..Biochem.[J],1993,32:10912—10922 [11] Lecomte S.,Wackerbarth H.,Soulimane T.et al..J.Am.Chem.Soc.[J],1998,120:7381—7382 [12] Munro O.Q.,Marques H.M..Inorg.Chem.[J],1996,35:3752—3767 [13] Santucci R.,Reinhard H.,BrunoriM..J.Am.Chem.Soc.[J],1988,110:8536—8537 [14] Jiang L.,Glidle A.,McNeil C.J.et al..Biosensors.Bioelectronics[J],1997,12:114321155 [15] Barkigia K.M.,Chantranupony L.,Smith K.M.et al..J.Am.Chem.Soc.[J],1988,110:7566—7567 [16] Alden R.G.,OndriasM.R.,Shelnutt J.A..J.Am.Chem.Soc.[J],1990,112:691—697 [17] Murray R.W.,Heineman W.R.,O′Dom G.W..Ana.lChem.[J],1967,39:1666—1668 [18] Palacios J.,Colina A.,Heras A.et al..Ana.lChem.[J],2001,73:2883—2889 [19] Wilson M.T.,Ranson R.J.,Masiakowski P.et al..Eur.J.Biochem.[J],1977,77:193—199 [20] Zhu Y.,Cheng G.,Dong S..Biophy.Chem.[J],2000,87:103—110 [21] Zhu Y.,Cheng G.,Dong S..Biophy.Chem.[J],2001,90:1—8 [22] Zhu Y.,Cheng G.,Dong S..J.Electrochem.Soc.[J],1999,146:3000—3003 [23] Reddy K.S.,Yonetani T.,Tsuneshige A.et al..Biochem.[J],1996,35:5562—5570 [24] Carraway A.D.,McCollum M.G.,Peterson J..Inorg.Chem.[J],1996,35:6885—6891 [25] Scholtz J.M.,Hong Q.,York E.J.et al..Biopolymers[J],1991,31:1463—1470 [26] Greenfield N.,Fasman G.D..Biochem.[J],1969,8:4108—4116 [27] Greenfield N..J.Biochem.[J],1981,20:33—37 [28] Melchionna S.,BarteriM.,Ciccotti G..J.Phys.Chem.[J],1996,100:19241—19250 [29] Kazanskaya I.,Lexa D.,BruschiM.et al..Biochem.[J],1996,35:13411—13418 [30] Blauer G.,Sreerama N.,Woody R.W..Biochem.[J],1993,32:6674—6679