高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (4): 636.

• 论文 • 上一篇    下一篇

IgA肾病免疫吸附剂的研究(Ⅱ)

褚佳强1, 俞耀庭1, 张卫华1, 陈建军1, 朱伯儒1, 刘景铎2   

  1. 1. 南开大学教育部生物活性材料重点实验室, 天津 300071;
    2. 北京空军总医院, 北京 100035
  • 收稿日期:2002-03-12 出版日期:2003-04-24 发布日期:2003-04-24
  • 通讯作者: 俞耀庭(1932年出生),男,教授,博士生导师,主要从事生物活性材料及酶工程的研究.E-mail:yaotE-maiing@pubE-mailic.tE-maipt.tE-maij.cn E-mail:E-mailE-mailic.tE-maipt.tE-maij.cn
  • 基金资助:

    国家自然科学基金(批准号:59703006)

In vitro Studies on the Immunoadsorbent for Removal of IgA in IgA-Nephropathy (Ⅱ)

CHU Jia Qiang1, YU Yao Ting1, ZHANG Wei Hua1, CHEN Jian Jun1, ZHU Bo Ru1, LIU Jing Duo2   

  1. 1. Key Lab of Bioactive Materials Research of Educational Ministry, Nankai University, Tianjin 300071;
    2. Air Force Military General Hospital, Beijng 100035, China
  • Received:2002-03-12 Online:2003-04-24 Published:2003-04-24

PDF (PC)

被引次数

28

摘要： 通过1,4-丁二醇-二缩水甘油醚法活化琼脂糖载体,既起到了活化载体的作用,同时又由于该活化剂分子是长链结构而给载体提供了一定长度的手臂,有利于人免疫球蛋白G(IgG)大分子配基与载体的连接,用该方法制成的免疫吸附剂对高IgA肾病病人血清中IgA进行了吸附研究.通过体外实验条件的优化发现,最高可吸附60%的IgA,同CDI法比较(吸附率可达35%左右),该吸附剂具有较高的吸附效率.

关键词: IgA肾病, 自身免疫疾病, 免疫吸附剂

Abstract: Bead type agarose as a carrier was prepared and activated by glycidyl 1,4 butanediol which could easily couple with human IgG because it has a long spacer, thus reducing the hinderence effect of IgG coupling reaction. In vitro experiments showed that the immunoadsorbent could adsorb 60% of IgA in plasma which is much higher than the CDI coupling method of 35%.

Key words: IgA nephropathy, Autoimmune disease, Immunoadsorbent

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