高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (6): 1143.

• 研究论文 • 上一篇    下一篇

电离辐射诱导T淋巴细胞的差异蛋白质组学研究

梁峰1, 石莹2, 张萱3, 刘忠英1, 刘志强2   

  1. 1. 吉林大学药学院, 长春 130021;
    2. 中国科学院长春应用化学研究所, 长春质谱中心, 长春 130022;
    3. 吉林大学公共卫生学院, 长春 130021
  • 收稿日期:2009-11-02 出版日期:2010-06-10 发布日期:2010-06-10
  • 通讯作者: 刘志强, 男, 博士, 研究员, 博士生导师, 主要从事天然药物化学与有机质谱学研究. E-mail: liuzq@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号: 20675079, 20873137, 30672600)资助.

Differential Proteomic Study of Human T Lymphocytic Leukemia Protein Induced by Ionizing Radiation

LIANG Feng1, SHI Ying2, ZHANG Xuan3, LIU Zhong-Ying1, LIU Zhi-Qiang2*   

  1. 1. School of PharmaceuticalSciences, Jilin University, Changchun 130021, China;
    2. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    3. School of Public Health, Jilin University, Changchun 130021, China
  • Received:2009-11-02 Online:2010-06-10 Published:2010-06-10
  • Contact: LIU Zhi-Qiang. E-mail: liuzq@ciac.jl.cn
  • Supported by:

    国家自然科学基金(批准号: 20675079, 20873137, 30672600)资助.

摘要:

应用电离辐射诱导人类T淋巴细胞白血病Jurkat细胞株, 再经二维凝胶电泳分离不同照射剂量的细胞总蛋白, 研究电离辐射诱导的人类T淋巴细胞白血病细胞蛋白的差异表达. 应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)进行处理, 得到肽质量指纹(PMF)图谱, 确认6个差异表达蛋白点.

关键词: 蛋白质组; 二维凝胶电泳; 基质辅助激光解吸电离飞行时间质谱; Jurkat细胞

Abstract:

Two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrorometry(MALDI-TOF-MS) have been acknowledged as the efficient methods in proteomics studies. In this report, the total proteins of human T lymphocytic leukemia(Jurkat cells) treated by ionizing radiation were separated with two-dimensional electrophoresis. The differential expression of cellular proteins induced by ionizing radiation was analyzed by PDQUEST software. Six differential protein spots were identified by MALDI-TOF-MS, including Lamin B1, heterogeneous nuclear ribonucleoprotein, p47 protein isoform a, alternative splicing factor 1, prohibitin, and 6-phosphogluconolactonase. The results may help researchers better understand the underlying mechanism involved in cellular protein alteration induced by ionizing radiation.

Key words: Proteome; Two-dimensional electrophoresis; Matrix-assisted laser desorption/ionization time-of-flight mass spectrorometry(MALDI-TOF-MS); Jurkat cell

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