基于Fe3O4@Au复合纳米粒子标记抗体的电化学免疫方法用于水体中大肠杆菌的检测

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (6): 1131.

基于Fe₃O₄@Au复合纳米粒子标记抗体的电化学免疫方法用于水体中大肠杆菌的检测

刘慧杰, 滕瑛巧, 张新爱, 符莹, 张文, 金利通

华东师范大学化学系, 上海 200062

收稿日期:2009-11-25 出版日期:2010-06-10 发布日期:2010-06-10
通讯作者: 张文, 男, 博士, 教授, 博士生导师, 主要从事生物电化学研究. E-mail: wzhang@chem.ecnu.edu.cn
基金资助:
国家自然科学基金(批准号: 20475017, 20775026)和上海市绿色化学与化工过程绿色化重点实验室开放基金资助.

Fe₃O₄@Au Nanoparticles as Labels for Electrochemical Immunodetection of E.coli in Water

LIU Hui-Jie, TENG Ying-Qiao, ZHANG Xin-Ai, FU Ying, ZHANG Wen*, JIN Li-Tong

Department of Chemistry, East China Normal University, Shanghai 200062, China

Received:2009-11-25 Online:2010-06-10 Published:2010-06-10
Contact: ZHANG Wen. E-mail: wzhang@chem.ecnu.edu.cn
Supported by:
国家自然科学基金(批准号: 20475017, 20775026)和上海市绿色化学与化工过程绿色化重点实验室开放基金资助.

摘要/Abstract

摘要：

合成了Fe₃O₄@Au复合纳米粒子作为辣根过氧化酶标记抗体的载体, 并将该复合纳米粒子标记物应用于电化学放大免疫分析. 将电子媒介体硫堇聚合在玻碳电极表面, 以纳米金作为固定大肠杆菌抗体的基底, 通过辣根过氧化酶催化溶液中H₂O₂产生的电流信号来测定大肠杆菌. 实验结果表明, 该方法对水体中大肠杆菌检测的线性范围为50～1×10⁵ cfu/mL, 检出限为20 cfu/mL. 对过富集后的实际水样进行测定, 该法结果表明, 对水体中大肠杆菌的检测灵敏度达到2 cfu/mL.

关键词: 大肠杆菌; Fe3O4@Au复合纳米粒子; 放大免疫法; 电化学免疫分析

Abstract:

Fe₃O₄@Au nanoparticles were synthesized in this paper. The surface of nanoparticles was modified with horseradish peroxidase(HRP)-anti-E.coli antibody for amplified electrochemical immunoassay of E.coli. Poly-thionine(PTH) film was electro-polymerized on the GCE as an electron-transfer mediator. Gold nanoparticles(GNPs) on the PTH film constructed an effective antibody immobilization matrix. Using the typical sandwich type immmunoassay, E.coli could be detected according to the H₂O₂ reduction currents catalyzed by HRP labeled on anti-E.coli antibody. The method allowed quantitative determination of E.coli concentration from 50 to 1×10⁵ cfu/mL, with a detection limit of 20 cfu/mL. By introducing pre-enrichment steps, a concentration of 2 cfu/mL E.coli was detected with this method.

Key words: E.coli; Fe3O4@Au nanoparticle; Amplified immunoassay; Electrochemical immunoassay

TrendMD:

刘慧杰, 滕瑛巧, 张新爱, 符莹, 张文, 金利通. 基于Fe₃O₄@Au复合纳米粒子标记抗体的电化学免疫方法用于水体中大肠杆菌的检测. 高等学校化学学报, 2010, 31(6): 1131.

LIU Hui-Jie, TENG Ying-Qiao, ZHANG Xin-Ai, FU Ying, ZHANG Wen*, JIN Li-Tong. Fe₃O₄@Au Nanoparticles as Labels for Electrochemical Immunodetection of E.coli in Water. Chem. J. Chinese Universities, 2010, 31(6): 1131.

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基于Fe₃O₄@Au复合纳米粒子标记抗体的电化学免疫方法用于水体中大肠杆菌的检测

Fe₃O₄@Au Nanoparticles as Labels for Electrochemical Immunodetection of E.coli in Water

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