cDNA基因芯片技术分析三聚氰胺肾毒性的相关基因表达

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (4): 684.

cDNA基因芯片技术分析三聚氰胺肾毒性的相关基因表达

时美¹, 范雪梅¹ , 李雪² , 王铮³ , 王义明² , 罗国安^1,2

1. 华东理工大学药学院, 上海 200237;
2. 清华大学中药现代化研究中心, 北京 100084;
3. 南开大学药学院, 天津 300071

收稿日期:2009-07-22 出版日期:2010-04-10 发布日期:2010-04-10
通讯作者: 罗国安, 男, 教授, 博士生导师, 主要从事中药分析方面研究. E-mail: luoga@tsinghua.edu.cn; 李雪, 女, 博士, 讲师, 主要从事基因方面的研究. E-mail: lixue@mail.tsinghua.edu.cn
基金资助:
国家“九七三”计划项目(批准号: 2007CB714505)、国家“十一五”科技支撑项目(批准号: 2006BAI08BO4-01)和教育部博士点基金(批准号: 20080031012)资助.

Gene Expression Studies of Melamine-related Renal Toxicity Based on cDNA Microarray

SHI Mei¹, FAN Xue-Mei¹, LI Xue^2* , WANG Zheng³, WANG Yi-Ming², LUO Guo-An^1,2*

1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
2. Modern Research Center of TCM, Tsinghua University, Beijing 100084, China;
3. School of Pharmacy, Nankai Universities, Tianjin 300071, China

Received:2009-07-22 Online:2010-04-10 Published:2010-04-10
Contact: LUO Guo-An. E-mail: luoga@tsinghua.edu.cn; LI Xue. E-mail: lixue@mail.tsinghua.edu.cn
Supported by:
国家“九七三”计划项目(批准号: 2007CB714505)、国家“十一五”科技支撑项目(批准号: 2006BAI08BO4-01)和教育部博士点基金(批准号: 20080031012)资助.

摘要/Abstract

摘要：

利用基因芯片技术筛查不同剂量的三聚氰胺干预大鼠肾脏的差异表达基因, 并对筛查的差异基因进行生物信息学分析, 推测三聚氰胺肾毒性的分子作用机制. 结果表明, 高剂量三聚氰胺干预的大鼠肾脏差异表达基因数多于低剂量干预的肾脏差异表达基因, 并且涉及到更多重要的分子功能和代谢途径, 表明三聚氰胺肾毒性具有剂量依赖性, 相比低剂量而言, 高剂量三聚氰胺干预对肾脏的危害更为严重.

关键词: 三聚氰胺; 基因芯片; 肾毒性; 肾结石

Abstract:

Nowadays, microarray technology has been used in toxicogenomics more and more often. In this paper, we applied it into the renal toxicity research of melamine, which is an intermediate of resin in chemical industry. The experimental, through microarray and bioinformatics, was designed to find the differences between two cases in which the rats independently intervened by high and low doses melamine, and then to interpret the molecular mechanism of renal toxicity. The result indicated that the number of differentially expressed genes intervened by high dose of melamine were more than that by low dose of melamine. Besides, the differential expressed genes interfered by high dose melamine have been proved to have close connection with some important pathways as follow: valine, leucine and isoleucine degradation, citrate metabolism, glutathione metabolism, fatty acid metabolism, and the molecular function such as mental binding, lyase activity, ligase activity, symporter activity, glutathione transferase activity, monooxygenase activity. Our research show the toxicity in kidney intervened by melamine, and demonstrated the special advantage of microarray technology in toxicogenomics.

Key words: Melamine; Microarray; Nephrotoxicity; Nephrolithiasis

TrendMD:

时美, 范雪梅 , 李雪 , 王铮 , 王义明 , 罗国安. cDNA基因芯片技术分析三聚氰胺肾毒性的相关基因表达. 高等学校化学学报, 2010, 31(4): 684.

SHI Mei, FAN Xue-Mei, LI Xue* , WANG Zheng, WANG Yi-Ming, LUO Guo-An* . Gene Expression Studies of Melamine-related Renal Toxicity Based on cDNA Microarray. Chem. J. Chinese Universities, 2010, 31(4): 684.

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