高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

[汤心颐先生诞辰100周年纪念专辑]丝素蛋白水凝胶的剪切诱导成胶动力学与机制

陆加霖1,2，庄雅灵1,3，王雅楠1,3，常  非2，丁建勋1,3，陈学思1,3   

  1. 1. 中国科学院长春应用化学研究所，高分子科学与技术全国重点实验室

    2. 吉林大学第二医院足踝外科 3. 中国科学技术大学，应用化学与工程学院

  • 收稿日期:2025-11-02 修回日期:2025-12-06 出版日期:2025-12-10 发布日期:2025-12-10
  • 通讯作者: 丁建勋 E-mail:jxding@ciac.ac.cn
  • 基金资助:
    国家重点研发计划(批准号：2022YFE0107700)，国家自然科学基金(批准号：U21A2099，82572761)和吉林省科技发展计划项目(批准号：20240101002JJ)

Shear-Induced Gelation Kinetics and Mechanisms of Silk Fibroin Hydrogels

LU Jialin1,2，ZHUANG Yaling1,3, WANG Yanan1,3, CHANG Fei2, DING Jianxun1,3, CHEN Xuesi1,3   

  1. 1. State Key Laboratory of Polymer Science and Technology,Changchun Institute of Applied Chemistry, Chinese Academy of Sciences 2. Department of Foot and Ankle Surgery,The Second Hospital of Jilin University 3. School of Applied Chemistry and Engineering,University of Science and Technology of China
  • Received:2025-11-02 Revised:2025-12-06 Online:2025-12-10 Published:2025-12-10
  • Contact: Jian-Xun DING E-mail:jxding@ciac.ac.cn
  • Supported by:
    Supported by the National Key R&D Program of China(No.2022YFE0107700), the National Natural Science Foundation of China(Nos.U21A2099 and  82572761) and the Science and Technology Development Program of Jilin Province, China(No.20240101002JJ)

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摘要： 本研究通过剪切-加热法诱导丝素蛋白形成富含β-折叠结构的水凝胶，系统考察了剪切时间、孵育温度及蛋白浓度对β-折叠结构组装动力学以及凝胶力学性能的影响。结果显示，0.03 g mL-1丝素蛋白溶液在10000 r min-1剪切10 min并于60 ℃孵育下，可在30 min内完成凝胶化，形成含63.51% β-折叠结构的物理交联网络，其储能模量达25.70 kPa、压缩强度为108.29 kPa。进一步揭示了丝素蛋白分子链由无规卷曲向β-折叠结构转变的自组装动力学特征及其力学性能演变规律。本研究为剪切诱导丝素蛋白水凝胶的制备工艺优化及其在组织工程中的应用提供了重要参考。

关键词: 丝素蛋白水凝胶, 剪切诱导, 二级结构转变, 成胶动力学, 生物医用材料

Abstract: In this study, a shear-heating method was employed to induce the formation of silk fibroin hydrogels enrich in β-sheet structures. The effects of shear duration, incubation temperature, and protein concentration on the kinetics of β-sheet assembly and the mechanical properties of the resulting hydrogels were systematically investigated. The results demonstrated that a 0.03 g mL-1 silk fibroin solution subjected to shear at 10000 r min-1 for 10 min and followed by incubation at 60 °C completed gelation within 30 min, yielding a physically cross-linked network containing 63.51% β-sheet structures, with a storage modulus of 25.70 kPa and a compressive strength of 108.29 kPa. Furthermore, the self-assembly kinetics of the molecular chain transition from random coils to β-sheet structures and the corresponding evolution of mechanical properties were elucidated. This work provides optimized processing strategies and experimental evidence for the preparation of shear-induced silk fibroin hydrogels and supports their potential applications in tissue engineering.

Key words: Silk fibroin hydrogel, Shear-inducing, Secondary structure transition, Gelation kinetics, Biomedical material

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