钝顶螺旋藻Spirulina platensis两种不同形态藻丝体的光合作用和蛋白质差异表达分析

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (6): 1152.

钝顶螺旋藻Spirulina platensis两种不同形态藻丝体的光合作用和蛋白质差异表达分析

林重阳, 沈晓文, 赵振坤, 吴韩志, 徐虹

厦门大学生命科学学院, 细胞生物学与肿瘤细胞工程教育部重点实验室, 厦门 361005

收稿日期:2008-08-11 出版日期:2009-06-10 发布日期:2009-06-10
通讯作者: 徐虹, 女, 博士, 副教授, 主要从事微藻生物化学与分子生物学研究. E-mail: hxu@xmu.edu.cn
基金资助:
国家自然科学基金(批准号: 30571446)和福建省高等学校新世纪人才支持计划资助.

Photosynthesis and Differentially Expressed Proteins in Two Morphologic Filaments of Spirulina platensis

LIN Chong-Yang, SHEN Xiao-Wen, ZHAO Zhen-Kun, WU Han-Zhi, XU Hong*

The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China

Received:2008-08-11 Online:2009-06-10 Published:2009-06-10
Contact: XU Hong, E-mail: hxu@xmu.edu.cn
Supported by:
国家自然科学基金(批准号: 30571446)和福建省高等学校新世纪人才支持计划资助.

摘要/Abstract

摘要：

从钝顶螺旋藻Spirulina platensis中分离纯化获得了螺旋形和直线形两种不同形态的藻丝体. 通过对二者光合作用的研究发现, 螺旋形藻丝体具有较高的光饱和光合作用速率(P_m^Chla)和光饱和点, 比直线形藻丝体更能适应较高光强的环境; 而直线形藻丝体具有较低的光补偿点, 能在更低的光强下进行光合作用. 通过双向凝胶电泳对两种不同形态藻丝体的总蛋白进行比较分析, 从中找出了9个差异表达的蛋白点. 应用基质辅助激光解吸电离质谱对差异表达的蛋白质点进行鉴定和数据库检索, 结果表明, 3个蛋白与肽聚糖代谢有关, 2个蛋白与光合作用有关, 1个蛋白与细胞分裂调控相关, 1个蛋白为外膜通道蛋白, 2个蛋白为功能未知的假想蛋白.

关键词: 钝顶螺旋藻, 形态差异, 光合作用, 双向电泳, 差异表达蛋白

Abstract:

The two kinds of different morphological filaments(spiral and linear ) of Spirulina platensis were separated and grown as axenic cultures. The photosynthetic characteristics show that compared with linear filaments, spiral filaments have higher light-saturated photosynthetic rate and light saturation point, and appeare to be more acclimated to high light intensity. Whereas linear filaments might be more acclimated to low light intensity for their lower light compensation point. To explore the morphologensis determination mechanism of Spirulina, the proteins that were differentially expressed in the spiral and the linear forms were studied. Two dimensional gel electrophoresis was performed to separate differentially expressed proteins that were subsequently identified by MALDI-TOF mass spectrometry. The 2-DE and PMF analysis revealed that at least nine proteins or subunits might relate to the morphogenesis of Spirulina platensis. The identified proteins include three proteins related to peptidoglycan metabolism, two photosynthetic proteins, one transmembrane channel protein, one protein related to cell division, as well as two unknown proteins.

Key words: Spirulina platensis, Morphogical transformation, Photosynthesis, Two-dimensional gel electrophoresis, Differentially expressed protein

TrendMD:

林重阳, 沈晓文, 赵振坤, 吴韩志, 徐虹. 钝顶螺旋藻Spirulina platensis两种不同形态藻丝体的光合作用和蛋白质差异表达分析. 高等学校化学学报, 2009, 30(6): 1152.

LIN Chong-Yang, SHEN Xiao-Wen, ZHAO Zhen-Kun, WU Han-Zhi, XU Hong*. Photosynthesis and Differentially Expressed Proteins in Two Morphologic Filaments of Spirulina platensis. Chem. J. Chinese Universities, 2009, 30(6): 1152.

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