高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (8): 1362-1367.doi: 10.7503/cjcu20170055

• 有机化学 • 上一篇    下一篇

有机农药异丙隆的级联生物降解体系的构建

王凤娇1, 李雪莲1, 胡满成1,2, 李淑妮1,2, 翟全国1,2, 蒋育澄1,2   

  1. 1. 陕西师范大学化学化工学院, 西安 710062;
    2. 大分子科学陕西省重点实验室, 西安 710062
  • 收稿日期:2017-01-20 修回日期:2017-07-12 出版日期:2017-08-10 发布日期:2017-07-12
  • 通讯作者: 蒋育澄,女,博士,教授,博士生导师,主要从事酶工程方面的研究.E-mail:jyc@snnu.edu.cn E-mail:jyc@snnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:21176150)和中央高校基本科研业务费(批准号:GK201701003)资助.

Cascade Biodegradation of Organic Pesticide Isoproturon

WANG Fengjiao1, LI Xuelian1, HU Mancheng1,2, LI Shuni1,2, ZHAI Quanguo1,2, JIANG Yucheng1,2   

  1. 1. School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China;
    2. Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi'an 710062, China
  • Received:2017-01-20 Revised:2017-07-12 Online:2017-08-10 Published:2017-07-12
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.21176150) and the Fundamental Research Funds for the Central Universities, China(No.GK201701003).

摘要:

构建了一个酶促氧化结合活性淤泥处理的级联生物降解有机农药异丙隆的体系.首先,建立了氯过氧化物酶(CPO)催化H2O2氧化降解异丙隆的酶处理体系.在最佳反应条件下,异丙隆的降解率可在10 min内达到100%.反应在室温下进行,pH=3.0,所需H2O2浓度为0.2 mmol/L,酶用量极少,仅7 nmol/L.降解体系高效、温和、绿色,无二次污染,极具应用潜力.CPO酶促氧化降解异丙隆的产物用HPLC-MS测定,并进一步根据主要产物推测了降解的途径.结果表明,异丙隆经CPO催化H2O2氧化处理后,被分解成较小的4-异丙基苯胺和4-乙基苯胺苄基正离子碎片分子.如果将CPO-H2O2酶促氧化作为一种前处理手段,后续与活性淤泥微生物降解过程结合,通过这种二级处理方法可实现含异丙隆污水的化学需氧量(COD)的有效去除,达到含异丙隆污水深度治理的目的.进一步以绿藻Chlorella pyrenoidosa的生长抑制率为评价指标对异丙隆酶催化氧化降解的产物进行了毒性评估,结果表明,随着底物降解率的逐渐增大,降解产物对绿藻的生长抑制率逐渐减小,说明经酶法降解后得到的产物与异丙隆相比毒性降低.

关键词: 氯过氧化物酶, 异丙隆, 级联生物降解, 产物检测, 毒性评估

Abstract:

Cascade biodegradation of organic pesticide isoproturon by enzymatic oxidation and activated sludge treatment is presented in this work. An enzymatic approach for the degradation of pesticide isoproturon catalyzed by chloroperoxidase with H2O2 as oxidant was proposed firstly. The complete degradation efficiency was achieved within 10 min at pH=3.0 and room temperature. The required H2O2 concentration was 0.2 mmol/L, and the dosage of chloroperoxidase(CPO) was only 7 nmol/L, indicating very few enzyme was required. The proposed degradation method was very efficient, mild, environment-friendly and no second pollution was involved, ensuring a potential application. The degradation products of isoproturon by CPO were determined by HPLC-MS, and the degraded route was proposed accordingly. Isoproturon was decomposed into small molecule 4-isopropyl aniline and 4-ethyl aniline benzylic cation by CPO-H2O2 catalytic oxidation. If the CPO-H2O2 was used as pre-stage treatment and was combined with activated sludge, the chemical oxygen demand(COD) can be eliminated to achieve effective decontamination of waste water containing isoproturon. Moreover, the ecotoxicity test using a green algae, Chlorella pyrenoidosa, showed that the growth inhibition ratio decreased with the increase of degradation of efficiency of isoproturon, indicating that the degraded products had lower toxicity than the parent isoproturon molecule.

Key words: Chloroperoxidase, Isoproturon, Cascade biodegradation, Products determination, Eco-toxicity evaluation

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