高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (8): 1368-1374.doi: 10.7503/cjcu20170127

• 有机化学 • 上一篇    下一篇

利用活体成像技术研究海茸β-1,3/1,6-葡聚糖在小鼠体内的分布

赵小亮, 刘曦, 杨忆, 蔡超, 吕友晶, 李国云, 郝杰杰, 于广利   

  1. 山东省糖科学与糖工程重点实验室, 中国海洋大学医药学院, 青岛 266003
  • 收稿日期:2017-03-02 修回日期:2017-06-02 出版日期:2017-08-10 发布日期:2017-06-02
  • 通讯作者: 郝杰杰,女,博士,副教授,主要从事药理学研究.E-mail:2009haojie@ouc.edu.cn;于广利,男,博士,教授,博士生导师,主要从事糖化学与糖药物学研究.E-mail:glyu@ouc.edu.cn E-mail:2009haojie@ouc.edu.cn;glyu@ouc.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:31670811),山东省科技重大专项(批准号:2015ZDJS04002)和泰山学者专项经费资助.

Detection of Animal Tissue Distribution of β-1,3/1,6-Glucan from Durvillaea antarctica by in vivo Imaging

ZHAO Xiaoliang, LIU Xi, YANG Yi, CAI Chao, LÜ Youjing, LI Guoyun, HAO Jiejie, YU Guangli   

  1. Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
  • Received:2017-03-02 Revised:2017-06-02 Online:2017-08-10 Published:2017-06-02
  • Supported by:

    Supported by the National Natural Science Foundation of China(No. 31670811), the Major Projects of Science and Technology in Shandong Province, China(No. 2015ZDJS04002) and the Program for Taishan Scholar of Shandong Province, China.

摘要:

以海茸β-1,3/1,6-葡聚糖(DAG)为研究对象,首先经还原胺化反应在DAG还原端引入功能氨基,再将其与N-羟基丁二酰亚胺(NHS)活化的荧光染料(Cy7)偶联,获得Cy7标记的DAG分子;利用小动物活体成像技术研究了DAG在小鼠体内的分布.结果表明,DAG在小鼠体内主要分布于肺、肝和肾,具有靶向小鼠肺部和通过肾排泄的特点.该方法简便、数据可靠,为多糖和寡糖的体内分布与代谢研究提供了参考.

关键词: β-1,3/1,6-葡聚糖, 荧光标记, 活体成像, 组织分布

Abstract:

In the present study, β-1,3/1,6-glucan(DAG) from Durvillaea antarctica was investigated. First, the functional amino group was introduced into the DAG reduction site by reductive amination reaction. The reaction product was then coupled with NHS-activated fluorescent dye(Cy7), and the Cy7-labeled DAG molecule was obtained subsequently. The distribution of DAG in mice was studied by small animal in vivo imaging technique. The results show that DAG is mainly distributed in the lungs, livers and kidneys, which possessed the characteristics of targeting the mouse lungs and excreting through the kidneys. The method is simple and reliable, which provides an important reference for the distribution and metabolism study of polysaccharides and oligosaccharides in vivo.

Key words: β-1,3/1,6-Glucan, Fluorescence labeling, In vivo imaging, Tissue distribution

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