基于表面重建螺旋型铂铱电极的葡萄糖生物传感器

doi:10.7503/cjcu20160777

高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (7): 1163.doi: 10.7503/cjcu20160777

基于表面重建螺旋型铂铱电极的葡萄糖生物传感器

潘帅, 李崭虹, 陈杨, 赵雪伶, 陈诚, 朱志刚()

上海第二工业大学工学部环境与材料工程学院, 上海 201209

收稿日期:2016-11-09 出版日期:2017-07-10 发布日期:2017-06-20
作者简介:联系人简介: 朱志刚, 男, 博士, 教授, 主要从事智能传感材料与器件研究. E-mail: zgzhu@sspu.edu.cn
基金资助:
国家自然科学基金(批准号: 61471233, 21504051)、上海高校特聘教授(东方学者)计划项目和上海市教委曙光计划项目(批准号: 14SG52)资助

Glucose Biosensor Based on Rebuilding the Surface of the Spiral-type Pt-Ir Electrode^†

PAN Shuai, LI Zhanhong, CHEN Yang, ZHAO Xueling, CHEN Cheng, ZHU Zhigang^*()

School of Environmental and Materials Engineering, College of Engineering,Shanghai Polytechnic University, Shanghai 201209, China

Received:2016-11-09 Online:2017-07-10 Published:2017-06-20
Contact: ZHU Zhigang E-mail:zgzhu@sspu.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(Nos 61471233, 21504051), the Program for Professor of Special Appointment(Eastern Scholar), China and the Shuguang Project of Shanghai Municipal Education Commission, China(No.14SG52)

摘要/Abstract

摘要：

通过对螺旋型铂铱电极表面进行化学腐蚀和电化学沉积铂纳米粒子实现电极表面的重建和优化, 研究了螺旋型铂铱电极在不同腐蚀时间和电沉积时间下的形貌及对过氧化氢(H₂O₂)的催化活性. 对表面重建的工作电极涂覆氧化酶和半透膜, 制备出了铂纳米粒子/葡萄糖氧化酶/环氧聚氨酯酶电极, 并将其用作葡萄糖传感器的工作电极. 传感器计时电流检测结果表明, 表面重建后的酶电极传感器对葡萄糖的检测范围扩大为2~45 mmol/L, 优于裸铂铱酶电极传感器, 电流响应值和灵敏度得到明显提升, 同时传感器还具有良好的稳定性和选择性.

关键词: 葡萄糖传感器, 螺旋型铂铱电极, 化学腐蚀, 电化学沉积, 检测范围

Abstract:

The surface of the spiral-type platinum-iridium electrode was rebuilt and optimized by chemical etching and electrochemical deposition of platinum nanoparticles(PtNPs). The effects of different etching time and electrodeposition time on the morphology and the catalytic activity of the electrode toward hydrogen peroxide were studied. After coating glucose oxidase(GOD) and epoxy-polyurethanes(Epoxy-PU) to surface-rebuilt electrode, the PtNPs/GOD/Epoxy-PU enzymatic biosensor was successfully fabricated. The chronoamperometric test indicates that the detection range of PtNPs/GOD/Epoxy-PU biosensor is expanded to 2—45 mmol/L, which is much wider than that of bare electrode based biosensor. PtNPs/GOD/Epoxy-PU biosensor has high response current and sensitivity, good stability and selectivity as well.

Key words: Glucose biosensor, Spiral-type platinum-iridium electrode, Chemical etching, Electrochemical deposition, Detection range(Ed.: S, Z, M)

中图分类号:

O657.1

TrendMD:

潘帅, 李崭虹, 陈杨, 赵雪伶, 陈诚, 朱志刚. 基于表面重建螺旋型铂铱电极的葡萄糖生物传感器. 高等学校化学学报, 2017, 38(7): 1163.

PAN Shuai, LI Zhanhong, CHEN Yang, ZHAO Xueling, CHEN Cheng, ZHU Zhigang. Glucose Biosensor Based on Rebuilding the Surface of the Spiral-type Pt-Ir Electrode^†. Chem. J. Chinese Universities, 2017, 38(7): 1163.

图/表 8

Fig.1 Schematic diagram of the spiral-type Pt-Ir electrode

Fig.2 Chronoamperometry responses of the spiral-type Pt-Ir electrode with different etching time to hydrogen peroxide(at 0.5 V vs. Ag/AgCl reference)(A), hydrogen peroxide sensitivity for the spiral-type Pt-Ir electrode with different etching time(B) and cyclic voltammograms of the spiral-type Pt-Ir electrode in 0.4 mmol/L K3(FeCN6)/0.1 mol/L KCl aqueous solution at 50 mV/s scanning rate with different etching time(C)Etching time/min: a. 0; b. 15; c. 30; d. 60.

Fig.3 SEM images of the spiral-type Pt-Ir electrodes Etching time/min: (A) 0; (B) 15; (C) 30; (D) 60.

Fig.4 Chronoamperometry responses of etched spiral-type Pt-Ir electrode with different electrodeposition time to hydrogen peroxide(at 0.5 V vs. Ag/AgCl)(A) and cyclic voltammograms of etched electrode in 0.1 mol/L H2SO4 aqueous solution at 50 mV/s scanning rate with different electrodeposition time(B) and hydrogen peroxide sensitivity for etched electrode with different electrodeposition time(C)

Table 1 Mass specific activity and active surface area of the spiral-type Pt-Ir electrode with different electrodeposition time

Etching time/min	Electro-deposition time/min	Deposition amount of platinum nanoparticles/mg	Active surface area/cm²	Activity surface area/ Deposition amount of Pt/(cm²·mg^-1)	Increased time of active surface area^*
0	0	0	0.039	0	0
30	5	0.28	0.489	1.75	12.54
30	10	0.41	0.786	1.92	19.15
30	15	0.79	1.026	1.30	25.30
30	20	1.11	1.070	0.96	26.44
30	25	1.50	1.435	0.95	36.80

Fig.5 SEM images of the etched spiral-type Pt-Ir electrode with various electrochemical deposition time Deposition time/min: (A) 0; (B) 5; (C) 10; (D) 15; (E) 20; (F) 25.

Fig.6 Amperometric response of etched PtNPs/GOD/Epoxy-PU electrode(a) and GOD/Epoxy-PU electrode(b) in different concentrations of glucose solution(A) and linear response curves of etched PtNPs/GOD/Epoxy-PU electrode(a) and GOD/Epoxy-PU electrode(b)(B)

Fig.7 Amperometric response(A) and sensitivity changes(B) of etched PtNPs/GOD/Epoxy-PU electrode within four weeks and sensor response to sequential additions of 5 mmol/L glucose, 0.5 mmol/L AA, 0.5 mmol/L DA, 0.5 mmol/L UA, 0.5 mmol/L F and 5 mmol/L glucose(C)

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基于表面重建螺旋型铂铱电极的葡萄糖生物传感器

Glucose Biosensor Based on Rebuilding the Surface of the Spiral-type Pt-Ir Electrode^†

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基于表面重建螺旋型铂铱电极的葡萄糖生物传感器

Glucose Biosensor Based on Rebuilding the Surface of the Spiral-type Pt-Ir Electrode†

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Glucose Biosensor Based on Rebuilding the Surface of the Spiral-type Pt-Ir Electrode^†