高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (2): 187-192.doi: 10.7503/cjcu20160721

• 分析化学 • 上一篇    下一篇

核酸适配体封盖的介孔二氧化硅纳米颗粒用于肌红蛋白的检测

高蕾, 王青, 羊小海(), 王柯敏(), 邓鹏, 张华, 李志平   

  1. 湖南大学化学生物传感与计量学国家重点实验室, 化学化工学院,生物纳米与分子工程湖南省重点实验室, 长沙 410082
  • 收稿日期:2016-10-17 出版日期:2017-02-10 发布日期:2017-01-16
  • 作者简介:联系人简介: 羊小海, 男, 博士, 教授, 博士生导师, 主要从事核酸分子探针及生物传感器方面的研究. E-mail: yangxiaohai@hnu.edu.cn;王柯敏, 男, 博士, 教授, 博士生导师, 主要从事纳米及分子水平上生物分析化学方面的研究. E-mail: kmwang@hnu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 21375034, 21675047, 21190040)和湖南省自然科学杰出青年基金(批准号: 2016JJ1008)资助

Aptamer-capped Mesoporous Silica Nanoparticles for Myoglobin Detection

GAO Lei, WANG Qing, YANG Xiaohai*(), WANG Kemin*(), DENG Peng, ZHANG Hua, LI Zhiping   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University,Changsha 410082, China
  • Received:2016-10-17 Online:2017-02-10 Published:2017-01-16
  • Contact: YANG Xiaohai,WANG Kemin E-mail:yangxiaohai@hnu.edu.cn;kmwang@hnu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21375034, 21675047, 21190040) and the Natural Science Foundation for Distinguished Young Scholars of Hunan Province, China(No.2016JJ1008)

摘要:

利用核酸适配体封盖的介孔二氧化硅纳米颗粒构建了一种新型、 简便及免标记的肌红蛋白定量检测方法. 首先, 用肌红蛋白核酸适配体将荧光小分子罗丹明6G封盖在介孔颗粒内, 当存在目标物肌红蛋白时, 由于介孔颗粒上的核酸适配体可特异性结合肌红蛋白而脱离介孔颗粒表面, 进而释放介孔颗粒内的罗丹明6G, 使溶液荧光强度增强. 实验结果表明, 荧光强度与肌红蛋白的浓度呈正相关, 通过荧光强度的变化可实现对肌红蛋白的定量检测. 该方法的检出限低至1.1 nmol/L, 且选择性好, 可满足临床医学的检测要求.

关键词: 核酸适配体, 肌红蛋白, 介孔二氧化硅纳米颗粒

Abstract:

A novel, simple and label free method was constructed for the quantitative detection of myoglobin based on the aptamer-capped mesoporous silica nanoparticles. Rhodamine 6G, a kind of small fluorescent molecules, was blocked in the aptamer-capped mesoporous silica particles. In the presence of myoglobin, the aptamer left from the mesoporous surface and the fluorescence intensity was significantly enhanced after releasing rhodamine 6G to the solution. The result showed that the concentration of myoglobin was positively associated with the fluorescence intensity of the solution. Therefore, the quantitative detection of myoglobin was achieved by monitoring the change of fluorescence intensity. Through this strategy, the limit of detection(LOD) of myoglobin was proved to be 1.1 nmol/L with excellent selectivity. It was suggested that the method could satisfactorily meet the detection requirement of myoglobin in clinical medicine.

Key words: Aptamer, Myoglobin, Mesoporous silica nanoparticles

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