信号核酸识体用于药物托普霉素的高灵敏度检测

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (5): 826.doi:

信号核酸识体用于药物托普霉素的高灵敏度检测

周翠松^1,2, 江雅新², 汪俊², 麻宝成², 李梦龙¹, 方晓红²

1. 四川大学化学学院, 成都 610064; 2. 中国科学院化学研究所, 北京 100080

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-05-10 发布日期:2006-05-10
通讯作者: 方晓红

High Sensitive Tobramycin Detection Using a Novel Signaling Aptamer

ZHOU Cui-Song ^{1, 2}, JIANG Ya-Xin², WANG Jun², MA Bao-Cheng², LI Meng-Long^1*, FANG Xiao-Hong ^2*

1. College of Chemistry, Sichuan University, Chengdu 610064, China;
2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10
Contact: FANG Xiao-Hong

摘要/Abstract

摘要：

提出了一种利用RNA核酸识体和光开关化合物[Ru(phen)₂dppz\]²⁺ (RU)高灵敏检测药物分子的荧光分析新方法. 以托普霉素(TOB)为靶分子, 检测限可达到10 nmol/L, 同时该方法有较好的选择性, 能在溶液中实现无需分离的实时检测. 这种无标记检测方法有利于不稳定RNA核酸识体在分析检测中的应用, 简化实验操作, 为其它药物分子的检测提供了新思路.

关键词: 核酸识体; 托普霉素; 荧光光谱法; 光开关化合物; 核糖核酸

Abstract:

Aptamers are a new class of oligonucleotides generated from in vitro selection with a high affinity and specificity. In this paper, a novel luminescence detection method was developed by using RNA aptamers and a DNA light switching complex, [Ru(phen)₂(dppz)]²⁺, as a signaling aptamer for the detection of drug tobramycin. The results showed that the method was highly sensitive and selective with the detection limit down to 10 nmol/L. The method was also very simple and could realize direct detection without separation in solution, and offers a new approach for the exploitation and application of aptamers in drug analysis.

Key words: Aptamer; Tobramycin; Luminescence spectroscopy; Light-switching complex; RNA

中图分类号:

O657.31
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O657.31

TrendMD:

周翠松,江雅新,汪俊,麻宝成,李梦龙,方晓红 . 信号核酸识体用于药物托普霉素的高灵敏度检测. 高等学校化学学报, 2006, 27(5): 826.

ZHOU Cui-Song ^{1, 2}, JIANG Ya-Xin², WANG Jun², MA Bao-Cheng², LI Meng-Long^1*, FANG Xiao-Hong ^2*. High Sensitive Tobramycin Detection Using a Novel Signaling Aptamer. Chem. J. Chinese Universities, 2006, 27(5): 826.