高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 484.
• Biological Analysis • 上一篇 下一篇
XIAO-Yao Xiao, DAVID McCalley
XIAO-Yao Xiao, DAVID McCalley
摘要:
A sensitive negative chemical ionization (NCI) gas-chromatography/mass spectrometry (GC/MS) method for the detection of estrogens is described. After hydrosis and clean-up by C18 ODS solid phase extraction (SPE) cartridge, the extracts obtained were derivatized with heptafluorobutyric anhydride and analyzed in the negative ion mode by GC/MS. Stability of derivatives was good. Selected ion monitoring (SIM) mode was applied to increase the sensitivity and, when possible, the higher m/z ions were selected to improve identification. The detection limit of the HFB esters in NCI using SIM was below 10 femtograms.
TrendMD: