高等学校化学学报

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (5): 834.doi:

• 研究论文 • 上一篇    下一篇

毛细管电泳乙腈-盐在线堆积方法机理研究

孔宇, 赵永席, 王波   

  1. 西安交通大学生物医学信息工程教育部重点实验室, 生命科学与技术学院生物技术与工程系, 西安 710049
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-05-10 发布日期:2006-05-10
  • 通讯作者: 孔宇

Studies on Mechanism of Acetonitrile-salts Stacking Method in Capillary Electrophoresis

KONG Yu*, ZHAO Yong-Xi, WANG Bo   

  1. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Bioengineering, School of Life Science and Technology, Xi′an Jiaotong University, Xi′an 710049, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10
  • Contact: KONG Yu

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被引次数

8

摘要：

以两种多肽为例, 在详细考察样品基质、 背景缓冲液性质以及其它操作因素对堆积影响的基础上, 提出乙腈-盐堆积法的可能机理为乙腈-致-快速-大体积堆积-盐诱导-类-等速电泳过程, 并用实验加以验证.

关键词: 毛细管电泳； 乙腈; 盐； 堆积； 机理

Abstract:

Acetonitrile-salt stacking method(ASS), as a new developed on-line stacking method in capillary electrophoresis, is suitable for stacking and analyzing low concentration analytes, which contain high concentrations of salts, and it has the huge practical potential in bio-analysis area. However, the applications are limited as the mechanism of ASS is not clear. In this work, the effects of sample matrix, experimental factors ect. on stacking were systematically studied. A new reasonable hypothesis about the mechanism of ASS was proved to be an “acetonitrile caused quick large volume stacking combined with NaCl induced pseudo isotachophoresis” procedure, and several experiments were carried out to improve the conclusion.

Key words: Capillary electrophoresis; Acetonitrile; Salt; Stacking; Mechanism

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