一种基于二氧化硅微颗粒的基因载体的制备新方法

高等学校化学学报 ›› 2006, Vol. 27 ›› Issue (5): 845.doi:

一种基于二氧化硅微颗粒的基因载体的制备新方法

林霞, 何晓晓, 王柯敏, 谭蔚泓

湖南大学化学生物传感与计量学国家重点实验室, 化学化工学院, 生物医学工程中心,
湖南省生物纳米工程技术研究中心, 长沙 410082

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-05-10 发布日期:2006-05-10
通讯作者: 王柯敏

A New Method for Preparation of Gene Carriers Based on Silica Micro-particles

LIN Xia, HE Xiao-Xiao, WANG Ke-Min^*, TAN Wei-Hong

State Key Laboratory of Chemo-Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Biomedical Engineering Center, Engineering Research Center for Bio-Nanotechnology of Hunan Province, Hunan University, Changsha 410082, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10
Contact: WANG Ke-Min

摘要/Abstract

摘要：

建立了一种基于二氧化硅微颗粒的基因载体的制备新方法. 首先将正硅酸乙酯在乙醇和氨水环境下水解，合成得到二氧化硅微颗粒, 然后通过静电作用将多聚赖氨酸修饰到硅微颗粒上, 制备出可有效地结合DNA的基因载体. 所制备的基因载体可将绿色荧光蛋白表达载体pEGFP导入COS-7细胞中, 实现了绿色荧光蛋白的高效表达. 本方法简便、快速, 在基因转染与基因治疗研究领域具有较好的潜在应用价值.

关键词: 二氧化硅微颗粒; 基因载体; 多聚赖氨酸

Abstract:

A new method for the preparation of gene carriers based on silica micro-particles(SiMPs) was reported. Silica micro-particles were synthesized by the hydrolysis of tetraethoxy\|silane in ethanol and ammonia, and then modified with poly-L-lysine(PLL) molecules via the electrostatic interaction to form PLL-SiMPs complexes as the gene carriers. These gene carriers can deliver pEGFP into COS-7 cells to accomplish GFP expression. This new method is simple and rapid and will be of great potential to be widely applied in gene transfection and gene therapy.

Key words: Silica micro-particle; Gene carrier; Poly-L-lysine

中图分类号:

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TrendMD:

林霞, 何晓晓, 王柯敏, 谭蔚泓 . 一种基于二氧化硅微颗粒的基因载体的制备新方法. 高等学校化学学报, 2006, 27(5): 845.

LIN Xia, HE Xiao-Xiao, WANG Ke-Min^*, TAN Wei-Hong. A New Method for Preparation of Gene Carriers Based on Silica Micro-particles. Chem. J. Chinese Universities, 2006, 27(5): 845.