希瓦氏菌介导制备还原氧化石墨烯薄膜及其增强的胞外电子传递

doi:10.7503/cjcu20140303

高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (10): 2201.doi: 10.7503/cjcu20140303

希瓦氏菌介导制备还原氧化石墨烯薄膜及其增强的胞外电子传递

张凯¹, 丁春梅¹, 刘欢¹(), 朱英¹(), 江雷^1,²

1. 北京航空航天大学仿生界面科学与技术教育部重点实验室, 化学与环境学院, 北京 100191
2. 中国科学院化学研究所, 北京分子科学国家实验室, 北京 100190

收稿日期:2014-04-02 出版日期:2014-10-10 发布日期:2014-09-19
作者简介:联系人简介: 朱英, 女, 博士, 教授, 博士生导师, 主要从事功能纳米材料及电化学性能研究. E-mail: zhuying@buaa.edu.cn。刘欢, 女, 博士, 副教授, 主要从事生物界面能量转化研究. E-mail: liuh@buaa.edu.cn。
基金资助:
国家自然科学基金(批准号: 51273008)、国家“八六三”计划项目(批准号: 2012AA030305)和国家“九七三”计划项目(批准号: 2012CB933200)资助

Shewanella-mediated Synthesis of Reduced Graphene Oxide Film for Enhanced Extracellular Electron Transfer^†

ZHANG Kai¹, DING Chunmei¹, LIU Huan^1,^*(), ZHU Ying^1,^*(), JIANG Lei^1,²

1. Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education,School of Chemistry and Environment, Behang University, Beijing 100191, China
2. Beijing National Laboratory for Molecular Sciences, Institute of Chemistry,Chinese Academy of Sciences, Beijing 100190, China

Received:2014-04-02 Online:2014-10-10 Published:2014-09-19
Contact: LIU Huan,ZHU Ying E-mail:liuh@buaa.edu.cn;zhuying@buaa.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.51273008), the National High Technology Research and Development Program of China(No.2012AA030305) and the National Basic Research Program of China(No.2012CB933200)

摘要/Abstract

摘要：

采用单室、三电极体系, 通过希瓦氏菌(Shewanella)还原氧化石墨烯(GO)膜, 制备了石墨烯(rGO)薄膜, 并通过X射线衍射(XRD)和拉曼光谱(Raman)等手段表征了GO还原前后的结构和性质, 证明了Shewanella菌可以有效将GO膜还原为rGO. 以该生物还原得到的rGO薄膜为工作电极, 可以有效改善Shewanella菌的胞外电子转移(EET). 与空白的氧化铟锡(ITO)电极相比, rGO电极的阳极输出电流增大了13%, 电量增加90%. 该Shewanella还原法是一种温和制备rGO膜的方法, 制得的rGO膜改善了微生物的EET效率.

关键词: 希瓦氏菌, 石墨烯电极, 外电子传递

Abstract:

A novel route was developed to prepare reduced graphene oxide(rGO) films through reduction of graphene oxide(GO) by Shewanella loihica PV-4(S. loihica PV-4) in a single-chamber, three-electrode system. Molecular structures of GO and rGO were characterized by X-ray diffraction and Raman spectroscopy. The results demonstrated that GO film was effectively reduced to rGO by S. loihica PV-4. The obtained rGO film was used as a work electrode to improve the extracllular electron transfer(EET) of S. loihica PV-4. Compared to ITO electrode, the anode current of rGO electrode with a film thickness of 111 nm increased by 13%, and the electric quantity increased by 90%. The Shewanella-mediated reducing method may offer a simple and mild route to prepare electrode material for high performance microbial fuel cell.

Key words: Shewanella, Graphene electrode, Extracellular electron transfer

中图分类号:

O646
O613.71

TrendMD:

张凯, 丁春梅, 刘欢, 朱英, 江雷. 希瓦氏菌介导制备还原氧化石墨烯薄膜及其增强的胞外电子传递. 高等学校化学学报, 2014, 35(10): 2201.

ZHANG Kai, DING Chunmei, LIU Huan, ZHU Ying, JIANG Lei. Shewanella-mediated Synthesis of Reduced Graphene Oxide Film for Enhanced Extracellular Electron Transfer^†. Chem. J. Chinese Universities, 2014, 35(10): 2201.

图/表 7

Fig.1 Sketch map of the experimental setup used in this study

Fig.2 Optical images of GO film(A) and rGO film(B) on ITO glass

Fig.3 SEM images of the as-prepared GO film (A) Surface section; (B) cross section.

Fig.4 C1s XPS spectra of GO(A) and rGO(B)

Fig.5 XRD patterns(A) and Raman spectra(B) of GO(a) and rGO(b)

Fig.6 Schematic illustration of bacterial EET on a GO film electrode

Fig.7 Current density vs. time curves at a constant potential of 0.2 V for S. loihica PV-4 on a bare ITO electrode(a) and GO film(b) with a thickness of 111 nm(A) and on GO membranes with thickness of 218 nm(a), 401 nm(b) and 856 nm(c)(B)

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希瓦氏菌介导制备还原氧化石墨烯薄膜及其增强的胞外电子传递

Shewanella-mediated Synthesis of Reduced Graphene Oxide Film for Enhanced Extracellular Electron Transfer^†

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希瓦氏菌介导制备还原氧化石墨烯薄膜及其增强的胞外电子传递

Shewanella-mediated Synthesis of Reduced Graphene Oxide Film for Enhanced Extracellular Electron Transfer†

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Shewanella-mediated Synthesis of Reduced Graphene Oxide Film for Enhanced Extracellular Electron Transfer^†