高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (5): 1084.doi: 10.7503/cjcu20130009

• 分析化学 • 上一篇    下一篇

三维荧光光谱结合二阶校正法测定保健品中的褪黑素

孙艳梅, 吴海龙, 王建瑶, 俞汝勤   

  1. 湖南大学化学生物传感与计量学国家重点实验室, 长沙 410082
  • 收稿日期:2013-01-05 出版日期:2013-05-10 发布日期:2013-05-10
  • 通讯作者: 吴海龙, 男, 博士, 教授, 博士生导师, 主要从事化学计量学方面的研究. E-mail: hlwu@hnu.edu.cn E-mail:hlwu@hnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 21175041)资助.

Determination of Melatonin in Functional Food by Three-dimensional Fluorescence Spectroscopy Combined with Second-order Calibration Method

SUN Yan-Mei, WU Hai-Long, WANG Jian-Yao, YU Ru-Qin   

  1. State Key Laboratory of Chemo/Biosensing Chemometrics, Hunan University, Changsha 410082, China
  • Received:2013-01-05 Online:2013-05-10 Published:2013-05-10

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被引次数

12

摘要：

采用三维荧光光谱结合基于交替三线性分解算法(ATLD)的二阶校正方法测定了2种保健品中褪黑素的含量. 当组分数取2时, 测得美益天褪黑素维生素B6胶囊(MYT)和纽斯葆牌褪黑素片(NSB)中褪黑素的含量分别为(3.074±0.019) mg/capsule和(1.649±0.059) mg/tablet, 平均加标回收率分别为(97.9±4.0)%和(103.4±2.7)%, 检出限为1.4 ng/mL. 结果表明, 该方法准确可靠. 该方法具有"二阶优势", 即使样品中存在干扰, 仍可获得较好的结果, 且灵敏度较高. 采用高效液相色谱(HPLC)法对该方法进行了验证, 用t-test对2种方法获得的结果进行比较发现, 2种方法的结果之间无显著性差异, 具有良好的一致性, 但本文方法更环保、 简单. 因此, 三维荧光光谱结合二阶校正方法可作为测定褪黑素含量的新方法, 用于对含褪黑素的保健类产品进行质量监控.

关键词: 三维荧光光谱, 二阶校正, 交替三线性分解, 二阶优势, 褪黑素, 保健食品

Abstract:

Three-dimensional fluorescence spectroscopy combined with second-order calibration method based on alternating trilinear decomposition(ATLD) algorithm was utilized to the determination of melatonin in functional food. When the number of spectral components was set to 2, the obtained content of melatonin in meiyitianTM melatonin and vitamin B6 capsule(MYT) and nature-power melatonin tablets(NSB) were (3.074±0.019) mg/capsule and (1.649±0.059) mg/tablet, and the average spiked recoveries were (97.9±4.0)% and (103.4±2.7)%, respectively, which demonstrated the reliability and accuracy of the established method. Owing to the second-order advantage achieved by the proposed method, satisfactory results can also be obtained even in the presence of interference(s). Additionally, high sensitivity was achieved because of the use of fluorescence spectroscopy. Besides, the method was also validated by high performance liquid chromatography(HPLC) with fluorescent detector, which is the conventional method for the determination of melatonin, and the results obtained by the two methods were compared by t-test and were found to be in good agreement with each other, but this method was environmentally friendly and easy. So the developed method can be an alternative method for the determination of melatonin and can be used to quality monitoring for functional food containing melatonin.

Key words: Three-dimensional fluorescence spectroscopy, Second-order calibration, Alternating trilinear decomposition, Second-order advantage, Melatonin, Functional food

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