高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

鹿茸寡肽的制备及其促成骨细胞的增殖作用

王华1,2, 林喆3, 刘强2, 蔡明军2, 徐力1, 张学忠1   

    1. 吉林大学分子酶学工程教育部重点实验室, 长春 130021;
    2. 吉林大学中日联谊医院, 长春 130033;
    3. 长春中医药大学中医药与生物工程研究开发中心, 长春 130117
  • 收稿日期:2008-02-29 修回日期:1900-01-01 出版日期:2008-09-10 发布日期:2008-09-10
  • 通讯作者: 张学忠, 徐力

Preparation of Velvet Antlers Small Peptides and Stimulating Effects on Osteosarcoma Cell Proliferation

WANG Hua1,2, LIN Zhe3, LIU Qiang2, CAI MING-Jun2, XU Li1*, ZHANG Xue-Zhong1*   

    1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, China;
    2. China-Japan Union Hospital of Jilin University, Changchun 130033, China;
    3. Research and Exploitation Center of Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China
  • Received:2008-02-29 Revised:1900-01-01 Online:2008-09-10 Published:2008-09-10
  • Contact: ZHANG Xue-Zhong, XU Li

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被引次数

47

摘要： 从梅花鹿鹿茸中提取了天然鹿茸总多肽(VATP). 在进一步分离纯化的过程中, 筛选出具有促进成骨细胞增殖的高活性肽组分(VAP-B), 并通过制备型HPLC对其纯化, 得到了分子量分布约为200~600的小肽活性组分(VAP-B2). 细胞周期分析结果表明, 鹿茸肽VAP-B2组分促进了人成骨肉瘤细胞OS-732的周期转化, 使其细胞周期S期细胞指数明显增加, 即表现为S期DNA含量明显提高. 碱性磷酸酶(ALP)活性检测结果表明, 随着鹿茸肽VAP-B2剂量的增加, ALP的活性明显增加. 这与成骨细胞增殖分化及成熟过程中, 细胞的周期性变化和骨形成标志物碱性磷酸酶水平的明显增高相符.

关键词: 梅花鹿鹿茸, 鹿茸肽, 分离与纯化, 生理活性, 成骨细胞

Abstract: The native total peptides(VATP)were extracted from velvet antlers of sika deer(Cerv us nippon Temminck). A high active peptide component(VAP-B)stimulating proliferation of a human osteosarcoma cells was isolated from VATP. Finally, a further purified active peptide component(VAP-B2) with molecular weight distribution from about 200—600 was obtained from VAP-B by preparative HPLC. VAP-B2 was able to enhance the average content of DNA in S phase. And also the alkali phosphatase(ALP) activity in osteosarcoma cells increased obviously with the increase of VAP-B2 dosage and treatment time. The results are consistent with cell cycle variation and ALP level as a marker of bond formation during osteocyte proliferation and differentiation, as well as maturity. The results obtained in this study provide a clue to explore a new peptide medicine from velvet antlers for therapy of osteoporosis.

Key words: Velvet antlers of sika deer, Sika deer peptides, Isolation and purification, Physiologic activity, Osteosarcoma cells

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