用反相离子对高效液相色谱法测定DNA中鸟嘌呤加胞嘧啶的百分含量

高等学校化学学报 ›› 1985, Vol. 6 ›› Issue (12): 1089.

用反相离子对高效液相色谱法测定DNA中鸟嘌呤加胞嘧啶的百分含量

都恒华, 张剑荣

南京大学化学系

收稿日期:1984-08-20 出版日期:1985-12-24 发布日期:1985-12-24
基金资助:
中国科学院科学基金

Determination of the Percentage of Guanine Plus Cytosine in Deoxyribonucleic Acid by Ion Pair Reversed Phase High Performance Liquid Chromatography

Du Henghua, Zhang Jianrong

Department of Chemistry, Nanjing University, Nanjing

Received:1984-08-20 Online:1985-12-24 Published:1985-12-24

摘要/Abstract

摘要： 本文提出了用反相离子对高效液相色谱测定脱氧核糖核酸(DNA)在88%甲酸水解产物中鸟嘌呤加胞嘧啶的摩尔百分含量的方法,用阶式梯度技术分离五种碱基。

Abstract: The percentage of guanine (G) plus cylosinc (C) in DNApreparations after acid hydrolysis in 88% formic acid is determined by ion pair reversed phase high performance liquid chromatography.By stepwisc gradient technique, uracil, cytosine, thymine, guanine, and adeninc are separated on a column of YWG -CH(C₁₈) with 2.5mM KH₂PO₄ buffer solution (pH=5.5) containing 5mMheptane sulfonate as the eluent(1), then with 2.5mM KH₂PO₄ buffer solution (pH=5.5) containing 18% methanol as the eluent(2).The results for the percentage determination of (G+C) are in close agreement with thermal melting point and buoyant density values determined for the DNAof calf thymus and sperm.

TrendMD:

都恒华, 张剑荣. 用反相离子对高效液相色谱法测定DNA中鸟嘌呤加胞嘧啶的百分含量. 高等学校化学学报, 1985, 6(12): 1089.

Du Henghua, Zhang Jianrong . Determination of the Percentage of Guanine Plus Cytosine in Deoxyribonucleic Acid by Ion Pair Reversed Phase High Performance Liquid Chromatography. Chem. J. Chinese Universities, 1985, 6(12): 1089.