高等学校化学学报

高等学校化学学报 ›› 1987, Vol. 8 ›› Issue (5): 418.

• 研究简报 • 上一篇    下一篇

同步荧光分析法同时测定叶绿素a和叶绿素b

黄贤智, 许金钩, 蔡挺   

  1. 厦门大学化学系
  • 收稿日期:1985-10-29 出版日期:1987-05-24 发布日期:1987-05-24
  • 基金资助:

    中国科学院科学基金

Simultaneous Determination of Chlorophyll a and Chlorophyll b by Synchronous Fluorimetry

Huang Xianzhi, Xu Jingou, Cai Ting   

  1. Department of Chemistry, Xiamen University, Xiamen
  • Received:1985-10-29 Online:1987-05-24 Published:1987-05-24

PDF (PC)

被引次数

29

摘要： 众所周知,叶绿素a和叶绿素b(分别用Chla和Chlb表示)在绿色植物的光合作用中起着极重要的作用。它们的测定通常用于不同波长处测量吸光度或在不同激发-发射波长下测量荧光强度,而后解联立方程的光度法。

Abstract: This paper reports a method for simultaneous determination of chlorophyll a and chlorophyll b by synchronous fluorimetry. The scanning wavelength intervals (△λ) of 258nm and 193nm were used and the wavelengths of maximum emission in synchroneous spectra were at 670nm and 654nm for chlorophyll a and chlorophyll b, respectively. This method is simple and rapid, and the ranges of determination were- 0.5-200 ppb. and 0.5-150 ppb, for chlorophyll a and chlorophyll b, respectively. Accuracy of deicrmination was about 5%.

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