高等学校化学学报

高等学校化学学报 ›› 1992, Vol. 13 ›› Issue (9): 1199.

• 论文 • 上一篇    下一篇

光化学-荧光分析法研究(Ⅸ)——现场光化学荧光分析法测定维生素K3注射液中甲萘醌含量

郭祥群, 许金钩, 赵一兵, 陈国珍   

  1. 厦门大学化学系, 厦门, 361005
  • 收稿日期:1991-09-21 出版日期:1992-09-24 发布日期:1992-09-24
  • 通讯作者: 郭祥群
  • 基金资助:

    国家自然科学基金

Studies on Photochemical Fluorimetric Method(Ⅸ)——Determination of Menadione in Vitamin K3 Injection by in situ Photochemical Fluorimetry

GUO Xiang-Qun, XU Jin-Gou, ZHAO Yi-Bing, CHEN Guo-Zhen   

  1. Department of Chemistry, Xiamen University, Xiamen, 361005
  • Received:1991-09-21 Online:1992-09-24 Published:1992-09-24

PDF (PC)

摘要： 自70年代以来,光化学荧光分析法(PCF)得到日益广泛的应用.PCF与HPLC或TLC联用具有独特的优点,尤其是在药物及复杂生物样品的分析方面显示出广阔的前景[1].但迄今为止,PCF的实施大多需要一个复杂的光化学反应装置[2].本文设计出一种简单易行的现场PCF技术,用同一光源的不同波长的光分别作光化学反应的光源和荧光测定的激发光,光照一定时间后,在产物的最大激发和发射波长处测量荧光强度,使光化学荧光分析能够在商品化的荧光分光光度计上进行.现已将此技术应用于维生素K3的分析,获得满意的结果.

关键词: 光化学反应, 荧光分析法, 维生素K3

Abstract: A method for determining menadione in vitamin K3 injection by in situ photochemical fluorimetry is reported in this paper.The effects of reaction medium,photon wavelength,oxygen,and irradiation time on the photoreduction reaction are also tested and discussed.Alinear curve is obtained over the rang of 0～9.60μg/mLmenadione (r=0.999) and the determination limit is 2.5 ng/mLfor menadione (w=11).The relative standard deviation is 2.7%.The a-mount of menadione in vitamin K3 injection determined by standard curve method and standard addition method is 94%～100% of marked amount.The recovery in standard addition test is 100%～107%.

Key words: Photochemical reaction, Fluorimetry, Vitamin K3

TrendMD: