高等学校化学学报

高等学校化学学报 ›› 1992, Vol. 13 ›› Issue (10): 1233.

• 论文 • 上一篇    下一篇

流动注射碱性降解荧光法快速测定微量金霉素

赵一兵, 余志雄, 郭祥群, 许金钩, 陈国珍   

  1. 厦门大学化学系, 厦门, 361005
  • 收稿日期:1991-10-19 修回日期:1992-06-22 出版日期:1992-10-24 发布日期:1992-10-24
  • 通讯作者: 赵一兵
  • 基金资助:

    国家自然科学基金

Rapid Alkaline Degradation Determination of Trace Chlorotetracycline by Flow Injection Fluorimetry

ZHAO Yi-Bing, YU Zhi-Xiong, GUO Xiang-Qun, XU Jin-Gou, CHEN Guo-Zhen   

  1. Department of Chemistry, Xiamen University, Xiamen, 361005
  • Received:1991-10-19 Revised:1992-06-22 Online:1992-10-24 Published:1992-10-24

PDF (PC)

被引次数

5

摘要： 提出了流动注射碱性降解荧光法快速测定微量金霉素的新方法.金霉素在流动管路中与0.25mol/LNaOH溶液快速反应,生成强荧光降解物.在流通池中用荧光法进行检测.反应产物荧光强度在10-8～10-5mol/L范围与金霉素含量成正比.共存四环素和脱水金霉素杂质不干扰金霉素测定.本法既可用于金霉素产品含量测定,又可用于四环素产品中杂质金霉素的测定,还成功地应用于尿液和血清中微量金霉素的测定.

关键词: 流动注射分析, 碱性降解, 荧光法, 金霉素

Abstract: The present paper covers a new flow injection fluorimetric method for the determination of trace chlorotetracycline.Chlorotetracycline reacts rapidly with 0.25 mol/L NaOHin a reaction tube and produces strong fluorescent species detected by fluorimetry in a flow cell.The fluorescence intensity of the product is proportional to the concentration of chlorotetracycline over the range of 10-8～10-5 mol/L.Coexisting tetracycline and anhydrochlorotetracycline do not interfere with the determination of chlorotetracycline.This method can be used not only for the quality detection of chlorotetracycline medicine but also for the determination of chlorotetracycline in tetracyline medicine.The determination of chlorotetracyciine in urine and serum is successful with satisfactory recoveries.

Key words: Flow injection analysis, Alkaline degradation, Fluorimetry, Chlorotetracycline

TrendMD: