高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (4): 897.

• 研究论文 • 上一篇    下一篇

低氧亲和力血红蛋白类氧载体的制备及大鼠动脉血压响应

严坤平1,2, 惠文利1, 罗超2, 谢于斗2, 彭明丽1,2, 陈超1,3   

  1. 1.  西北大学生命科学学院, 西安 710069;
    2.  陕西北美基因股份有限公司, 西安 710069; 
    3.  国家微检测工程研究中心, 西安 710069
  • 收稿日期:2010-05-17 修回日期:2010-11-10 出版日期:2011-04-10 发布日期:2011-03-09
  • 通讯作者: 严坤平,陈超 E-mail:kpyan@nwu.edu.cn;chaochen@nwu.edu.cn
  • 基金资助:

    国家“八六三”计划项目(批准号:  2006AA02A143)和陕西省教育厅专项科研计划项目(批准号:  09JK784)资助.

Preparation of Hemoglobin-based Oxygen Carrier with Low Oxygen Affinity and Arterial Blood Pressure Responses in Rat

YAN Kun-Ping1,2*, HUI Wen-Li1, LUO Chao2, XIE Yu-Dou2, PENG Ming-Li1,2, CHEN Chao1,3*   

  1. 1. College of Life Science, Northwest University, Xi′an 710069, China;
    2. Shanxi Lifegen Co., Ltd., Xi′an 710069, China;
    3. National Engineering Research Center for Miniaturized Detection System, Xi′an 710069, China
  • Received:2010-05-17 Revised:2010-11-10 Online:2011-04-10 Published:2011-03-09
  • Contact: YAN Kun-Ping, CHEN Chao E-mail:kpyan@nwu.edu.cn;chaochen@nwu.edu.cn
  • Supported by:

    国家“八六三”计划项目(批准号:  2006AA02A143)和陕西省教育厅专项科研计划项目(批准号:  09JK784)资助.

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被引次数

5

摘要： 氧亲和力是血红蛋白氧载体非常重要的参数之一，但其高低还没有统一的认识，有一种理论认为低氧亲和力是造成高血压的原因。为了进一步研究氧亲和力与血压之间的关系，本文制备了两种低氧亲和力的血红蛋白氧载体。用高碘酸钠氧化棉子糖（高碘酸钠：棉籽糖=6：1，摩尔比），得到氧化均一的开环棉子糖；以其作为交联剂聚合脱氧猪血红蛋白，在聚合1 h得到了低氧亲和力（P50 = 43.1 torr）主要为分子内交联的64 kDa的血红蛋白，在聚合2 4 h得到了低氧亲和力（P50 = 51.5 torr）平均分子量为600 kDa的聚合血红蛋白；分别进行大鼠50%等容量换血实验，前者显著引起血压升高而后者血压平稳，证实高血压主要和64 kDa血红蛋白有关，低氧亲和力不是造成高血压的主要原因。

关键词: 猪血红蛋白, 棉籽糖, 血红蛋白氧载体, 氧亲和力, 高血压

Abstract: The oxygen affinity are one of important parameters of hemoglobin-based oxygen carriers(HBOCs), and are not completely understand at present. One theory considered that the low oxygen affinity are the main reason for the hypertension. In order to further research the relationship between the oxygen affinity and the hypertension, in this research, two Hemoglobin-based oxygen carriers(HBOCs) with low oxygen affinity were prepared. The raffinose were oxidized by sodium periodate (sodium periodate:raffinose=6:1, molar ratio), yielding a completely oxidized and homogeneous polyaldehyde ring-opened raffinose. It was used as the cross-linker to polymerize porcine deoxyhemoglobin, for 1 h to yield a low oxygen affinity (P50=43.1 torr) and mainly intro-molecular cross-linked 64 kDa hemoglobin(pCLHb); for 24 to yield a low oxygen affinity (P50=51.5 torr) and mean molecular weight 600 kDa polymerized hemoglobin(pPolyHb). With the two kind of HBOCs, 50% isovolemic exchange transfusion were performed in rat, the pCLHb caused an immediate and sustained hypertension but pPolyHb didn’t, and indicated hypertension are related to the 64 kDa hemoglobin and low oxygen affinity aren’t the main reason for hypertension.

Key words: Porcine hemoglobin, Raffinose, Hemoglobin-based oxygen carrier, Oxygen affinity, Hypertension

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