高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (4): 863.

• 研究论文 • 上一篇    下一篇

ESI-MS研究N-苯甲酰基-脱氢枞胺衍生物与血管紧张肽Ⅲ的相互作用

吕林1,邓慧敏1,林中祥2   

  1. 1. 中山大学测试中心, 广州 510275;
    2. 南京林业大学化学工程学院,  南京 210037
  • 收稿日期:2010-06-08 修回日期:2010-09-22 出版日期:2011-04-10 发布日期:2011-03-09
  • 通讯作者: 邓慧敏 E-mail:denghm@mail.sysu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:  30771688,  30871989)资助.

Interaction Between N-Benzoyl\|dehydroabietylamine Derivatives with Angiotensin Ⅲ by Electrospray  Ionization Mass Spectrometry

LÜ Lin1, DENG Hui-Min1*, LIN Zhong-Xiang2   

  1. 1. Instrumental Analysis Research Center, Sun Yat\|sen University, Guangzhou 510275, China;
    2. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
  • Received:2010-06-08 Revised:2010-09-22 Online:2011-04-10 Published:2011-03-09
  • Contact: DENG Hui-Min E-mail:denghm@mail.sysu.edu.cn
  • Supported by:

    国家自然科学基金(批准号:  30771688,  30871989)资助.

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被引次数

16

摘要： 采用电喷雾质谱法,考察了4种最新合成的N-苯甲酰基-脱氢枞胺衍生物（NBDD）——N-苯甲酰基-脱氢枞胺(NBD),N-邻氯苯甲酰基-脱氢枞胺(NClBD)，N-苯甲酰基-脱氢枞胺-7-酮(NBDO)，N-邻氯苯甲酰基-脱氢枞胺-7-酮(NClBDO)与具有重要生理作用的血管紧张肽Ⅲ（AngⅢ）的相互作用。结果表明,这4种化合物与AngⅢ以不同的摩尔比 (1:1,3:1,5:1)混合时，均可形成非共价复合物，并主要形成+2价复合物离子。对4种NBDD:AngⅢ为1:1的+2价复合物离子[NBDD-AngⅢ+2H]2+进行了2级质谱分析,并根据相对碰撞能的大小,比较了这4种复合物离子的稳定性。对NBDD:AngⅢ为2:1的+2价复合物离子[(NBDD)2-AngⅢ+2H]2+进行了2级和3级质谱分析，并探讨了(NBDD)2-AngⅢ复合物中NBDD分子与AngⅢ的结合方式。

Abstract: The interactions between the novel N-benzoyl-dehydroabietylamine derivatives（NBDD）including N-benzoyl-dehydroabietylamine(NBD),N-o-chlorobenzoyl-benzoyl–dehydroabietyl- amine(NClBD),N-benzoyl-dehydroabietylamine-7-one(NBDO),N-o-chlorobenzoyl–dehydroabi- etylamine-7-one(NClBDO) with Angiotensin Ⅲ were investigated by electrospray ionization mass spectrometry (ESI-MS). The results showed that the four derivatives all could form non-covalent complexes with Ang Ⅲ when the derivatives mixed with Ang Ⅲ in molar ratio of NBDD:AngⅢ 1:1, 3:1,5:1, and the complexes mainly formed +2 charged ions. The MS2 of [NBDD-AngⅢ+2H]2+ and MS2 and MS3 of [(NBDD)2-AngⅢ+2H]2+ were studied．The stability of four kinds of complexes [NBDD-AngⅢ+2H]2+ and the combination mode of NBDD with Ang Ⅲ were discussed.

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