高等学校化学学报

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (11): 2680.

• 研究论文 • 上一篇    下一篇

(PAA-b-F127-b-PAA)-Blg复合物的合成及乳化性能

孟令敏1,4, 梁蓉2, 石珂3, 黄庆荣3, 石彤非1   

  1. 1. 中国科学院长春应用化学研究所, 高分子物理与化学国家重点实验室, 长春 130022;]2. 江南大学食品学院, 食品科学与技术国家重点实验室, 无锡 214122;
    3. Department of Food Science, Rutgers University, New Brunswick, New Jersey 08901, USA;
    4. 中国科学院研究生院, 北京 100049
  • 收稿日期:2010-12-23 修回日期:2011-04-25 出版日期:2011-11-10 发布日期:2011-10-14
  • 通讯作者: 石彤非 E-mail:tfshi@ciac.jl.cn
  • 基金资助:

    国家自然科学基金海外及港澳学者合作研究基金(批准号: 51028301)和国家自然科学基金创新研究群体科学基金(批准号: 50621302)资助.

Synthesis of (PAA-b-F127-b-PAA)-β-lactoglobulin Conjugate with Improved Emulsifying Properties

MENG Ling-Min1,4, LIANG Rong2, SHI Ke3, HUANG Qing-Rong3, SHI Tong-Fei1*   

  1. 1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
    3. Department of Food Science, Rutgers University, New Brunswick, New Jersey 08901, USA;
    4. Graduate School of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2010-12-23 Revised:2011-04-25 Online:2011-11-10 Published:2011-10-14
  • Contact: SHI Tong-Fei E-mail:tfshi@ciac.jl.cn
  • Supported by:

    国家自然科学基金海外及港澳学者合作研究基金(批准号: 51028301)和国家自然科学基金创新研究群体科学基金(批准号: 50621302)资助.

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被引次数

3

摘要： 采用原子转移自由基聚合(ATRP)方法合成了PtBA-b-F127-b-PtBA(PtBA为聚丙烯酸叔丁酯), 经三氟乙酸水解得到PAA-b-F127-b-PAA(PAA为聚丙烯酸). 进一步通过碳二亚胺的水溶性衍生物1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC?HCl)在水溶液中成功地耦合β-乳球蛋白(Blg)和PAA-b-F127-b-PAA. 蛋白的三级构象没有受到反应条件的影响. 与纯Blg相比, (PAA-b-F127-b-PAA)-Blg复合物的乳化性能大大提高.

关键词: 氧化乙烯-氧化丙烯-氧化乙烯嵌段共聚物, 聚丙烯酸, β-乳球蛋白复合物, 乳化性能

Abstract: Poly(acrylic acid)-b-F127-b-poly(acrylic acid)(PAA-b-F127-b-PAA) was synthesized through atom transfer radical polymerization(ATRP) in combination with the subsequent hydrolysis of tert-butyl groups using excess trifluoroacetic acid(TFA). The (PAA-b-F127-b-PAA)-β-lactoglobulin conjugate was successfully synthesized using water soluble 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride(EDC?HCl), a carbodiimide derivative, as the conjugation agent. Fluorescence spectroscopy indicated that the ternary structure of protein was maintained even conjugation to PAA-b-F127-b-PAA. The conjugate exhibited significantly better emulsifying properties than that of pure β-lactoglubulin.

Key words: Pluronic, Poly(acrylic acid), β-Lactoglobulin conjugate, Emulsifying property

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