双阳离子咔唑衍生物与DNA相互作用的光谱研究

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (9): 1860.

双阳离子咔唑衍生物与DNA相互作用的光谱研究

张元红¹, 孙渝明², 刘鑫¹, 赵宁¹,于晓强¹, 黄柏标¹

1. 山东大学晶体材料国家重点实验室,
2. 山东大学光学系, 济南 250100

收稿日期:2010-06-07 出版日期:2010-09-10 发布日期:2010-09-10
通讯作者: 于晓强. E-mail: yuxq@sdu.edu.cn; 黄柏标. E-mail: bbhuang@sdu.edu.cn
基金资助:
国家自然科学基金(批准号: 50673053, NSFC/RGC50218001, 50173015, 20973102)资助.

Interaction Between Dicationic Carbazole Derivatives and Nucleic Acid by Spectrum

ZHANG Yuan-Hong¹, SUN Yu-Ming², LIU Xin¹, ZHAO Ning¹, YU Xiao-Qiang^1*, HUANG Bai-Biao^1*

1. State Key Laboratory of Crystal Materials,
2. Optics Department, Shandong University, Jinan 250100, China

Received:2010-06-07 Online:2010-09-10 Published:2010-09-10
Contact: YU Xiao-Qiang. E-mail: yuxq@sdu.edu.cn; HUANG Bai-Biao. E-mail: bbhuang@sdu.edu.cn
Supported by:
国家自然科学基金(批准号: 50673053, NSFC/RGC50218001, 50173015, 20973102)资助.

摘要/Abstract

摘要： 合成了一种新型的双阳离子咔唑衍生物3,6-双(1-羟乙基-4-烯基吡啶)咔唑碘盐(3,6-BHVC), 并对其进行了表征. 利用紫外-可见吸收光谱、圆二色谱、单光子和双光子荧光发射光谱探讨了该化合物与DNA的结合方式. 所有光谱测试结果表明, 该化合物在[磷酸根]/[染料]比值较低的条件下主要以插入方式与DNA结合, 在该比值较高的条件下主要以沟槽结合方式为主. 表明咔唑衍生物可以成为一种探索具有高结合能力双光子DNA探针的基本结构.

关键词: 双阳离子咔唑衍生物, 双光子细胞核荧光探针, 结合方式

Abstract: In this paper, 3,6-bis(1-hydroxyethyl-4-vinylpyridinium) carbazole diiodide(3,6-BHVC), a novel dicationic carbazole derivative, was synthesized and characte-rized. Using electronic absorption spectra, circle dichroism spectra and one- and two-photon emission spectra, the binding modes between 3,6-BHVC and DNA were studied. All spectral results show that 3,6-BHVC intercalates into DNA base pairs at lower ratio of [phosphate]/[3,6-BHVC] and binds in DNA groove at higher ratio. This indicates that carbazole derivative can act as a primary structure to explore two-photon DNA probes with high binding affinity.

Key words: Dicationic carbazole derivative, Two-photon fluorescent DNA probe, Binding mode

TrendMD:

张元红, 孙渝明, 刘鑫, 赵宁,于晓强, 黄柏标. 双阳离子咔唑衍生物与DNA相互作用的光谱研究. 高等学校化学学报, 2010, 31(9): 1860.

ZHANG Yuan-Hong, SUN Yu-Ming, LIU Xin, ZHAO Ning, YU Xiao-Qiang*, HUANG Bai-Biao. Interaction Between Dicationic Carbazole Derivatives and Nucleic Acid by Spectrum. Chem. J. Chinese Universities, 2010, 31(9): 1860.

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