溶剂对脂肪酶降解高分子量PBS及改性共聚物的影响

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (3): 612.

溶剂对脂肪酶降解高分子量PBS及改性共聚物的影响

张敏^1,2, 丁明亮¹, 张婷¹, 杨金明¹

1. 陕西科技大学教育部轻化工助剂化学与技术重点实验室, 西安 710021;
2. 浙江温州轻工研究院, 温州 325003

收稿日期:2009-05-31 出版日期:2010-03-10 发布日期:2010-03-10
通讯作者: 张敏, 女, 博士, 教授, 博士生导师, 主要从事环境友好型高分子材料的合成、应用及基础理论研究. E-mail: zhangmin@sust.edu.cn
基金资助:
国家自然科学基金(批准号: 50673056)、温州科技计划项目(批准号: H20080045)和陕西省重大科技创新项目(批准号: 2009ZKC08-09)资助.

Effect of Solvent on the Enzyme Catalysis Biodegradation of PBS with High Molecular Weight and Its Modified Copolymer

ZHANG Min^1,2*, DING Ming-Liang¹, ZHANG Ting¹, YANG Jin-Ming¹

1. Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science and Technology, Xi′an 710021, China;
2. Zhejiang Wenzhou Research Institute of Light Industry, Wenzhou 325003, China

Received:2009-05-31 Online:2010-03-10 Published:2010-03-10
Contact: ZHANG Min. E-mail: zhangmin@sust.edu.cn
Supported by:
国家自然科学基金(批准号: 50673056)、温州科技计划项目(批准号: H20080045)和陕西省重大科技创新项目(批准号: 2009ZKC08-09)资助.

摘要/Abstract

摘要：

采用固定化脂肪酶分别在四氢呋喃(THF)、甲苯及THF和甲苯的混合溶剂体系中, 对高分子量的PBS及PBS/CHDM共聚物进行了催化降解, 用GPC测定了降解产物的分子量, 用质谱(MS)对降解产物进行了分析, 用FTIR表征了产物化学结构. 研究结果表明, 在脂肪酶的作用下, 对M_n在10⁵以上的PBS, 在THF体系中可降解到1.4×10⁴, 而在甲苯体系中可降解到4×10⁴; 但在THF和甲苯的混合体系中, 可将M_n 10⁵以上的PBS和PBS/CHDM共聚物都可降解为0.1×10⁴以下的原料单体、一至四环状低聚物及少量线型低聚物. 分析了水含量对酶解反应的影响.

关键词: 脂肪酶降解; PBS改性共聚物; 原料单体; 环状低聚物; 线型低聚物

Abstract:

PBS and PBS/CHDM copolymers with high molecular weight were degraded via immobilized lipase in different polar solvents, such as toluene, tetrahydrofuran, and mixed solvent of toluene and tetrahydrofuran, respectively. The degradation products were analyzed by mass spectrometry(MS). The chemical structure was characterized by FTIR. And the molecular weight was determined by GPC. Under the function of immobilized lipase, PBS with M_n=100000 can be degraded to M_n=40000 in toluene and to M_n=14000 in tetrahydrofuran system. While in mixed solvent of toluene and tetrahydrofuran, PBS and PBS/CHDM with M_n more than 100000 can be degraded to below M_n=1000. The products almost are monomer, 1—4 cyclic oligomer, liner oligomer and a small amount of linear oligomer. The existence of a small amount of water has a certain impact on enzymatic reaction.

Key words: Lipase degradation; Modified copolymer of PBS; Monomer; Cyclic oligomer; Linear oligomer

TrendMD:

张敏, 丁明亮, 张婷, 杨金明. 溶剂对脂肪酶降解高分子量PBS及改性共聚物的影响. 高等学校化学学报, 2010, 31(3): 612.

ZHANG Min*, DING Ming-Liang, ZHANG Ting, YANG Jin-Ming. Effect of Solvent on the Enzyme Catalysis Biodegradation of PBS with High Molecular Weight and Its Modified Copolymer. Chem. J. Chinese Universities, 2010, 31(3): 612.