高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (1): 61.

• 研究快报 • 上一篇    下一篇

基于铁薄膜的表面等离子体耦合定向荧光研究

曹烁晖, 谢堂堂, 蔡伟鹏, 李耀群   

  1. 厦门大学化学化工学院化学系与现代分析科学重点实验室, 厦门361005
  • 收稿日期:2009-10-15 出版日期:2010-01-10 发布日期:2010-01-10
  • 通讯作者: 李耀群, 女, 博士, 教授, 博士生导师, 主要从事分子发光研究. E-mail: yqlig@xmu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 20975084, 20575055)和国家“九七三”计划项目(批准号: 2007CB935600)资助.

Observation of Surface Plasmon-coupled Directional Fluorescence Using Thin Iron Films

CAO Shuo-Hui, XIE Tang-Tang, CAI Wei-Peng, LI Yao-Qun*   

  1. Department of Chemistry and Key Laboratory of Analytical Sciences College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
  • Received:2009-10-15 Online:2010-01-10 Published:2010-01-10
  • Contact: LI Yao-Qun. E-mail: yqlig@xmu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20975084, 20575055)和国家“九七三”计划项目(批准号: 2007CB935600)资助.

摘要:

本文报道了以纳米铁膜为基底的SPCDF检测方法. 以荧光素和卟啉为例, 探讨了SPCDF信号的特性, 在固定角度下检测了荧光素-卟啉双荧光团体系的SPCDF信号, 并应用于全血样品的分析.

关键词: 表面等离子体耦合定向荧光; 铁薄膜; 定向角; 双荧光团

Abstract:

The observation of surface plasmon-coupled directional fluorescence(SPCDF) on thin iron films was presented. SPCDF from thin iron films was p-polarized with a directional emission angle of 70°. Fluorescein and meso-tetra(4-sulfonatophenyl)porphine(TPPS) were used as a model system of dual fluorophores. Using 25 nm thin iron films, the SPCDF signals of the two fluorophores were observed at a fixed angle just by one scan. The SPCDF signals of Fluorescein and TPPS added to whole blood were identified clearly, eliminating the background interference of blood effectively. Thin iron films have been proven to be new materials for SPCDF detection, promoting a new mode to observe the enhanced fluorescence signals of different fluorophores by one scan. They should be useful for tracking the labled systems of multi-fluorophores in biological applications.

Key words: Surface plasmon-coupled directional fluorescence(SPCDF); Thin iron film; Directional emission angle; Dual fluorophore

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