高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (8): 1577.

• 研究论文 • 上一篇    下一篇

乙酰鸟氨酸脱乙酰酶固定化细胞拆分D,L-缬氨酸

张飞1,2, 熊吉滨1, 刘均忠1, 刘鹏刚1, 焦庆才1   

  1. 1. 南京大学生命科学学院, 医药生物技术国家重点实验室, 南京 210093;
    2. 包头轻工职业技术学院生物工程系, 包头 014043
  • 收稿日期:2008-12-30 出版日期:2009-08-10 发布日期:2009-08-10
  • 通讯作者: 焦庆才, 男, 博士, 教授, 博士生导师, 从事氨基酸及其衍生物的化学生物合成研究. E-mail: jiaoqc@yahoo.com.cn
  • 基金资助:

    国家技术创新基金(批准号: 02CJ-13-01-16) 资助.

Chiral Resolution of D,L-Valine by Immobilized Cells with Acetylornithine Deacetylase Activity

ZHANG Fei1,2, XIONG Ji-Bing1, LIU Jun-Zhong1, LIU Peng-Gang1, JIAO Qing-Cai1*   

  1. 1. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China;
    2. Baotou Light Industry Vocational Technical College, Baotou 014043, China
  • Received:2008-12-30 Online:2009-08-10 Published:2009-08-10
  • Contact: JIAO Qing-Cai. E-mail: jiaoqc@yahoo.com.cn
  • Supported by:

    国家技术创新基金(批准号: 02CJ-13-01-16) 资助.

摘要:

报道了一种利用具有乙酰鸟氨酸脱乙酰酶活性的固定化细胞拆分D,L-缬氨酸的新方法. 该酶促反应最适条件: pH=6, 反应温度50 ℃, 底物N-乙酰-D,L-缬氨酸浓度200 mmol/L, 固定化细胞用量0.2 g/mL(或100 U/mL). 0.1 mmol/L CoCl2条件对该酶促反应有显著的激活作用. 在以上条件下反应2~3 h, 测得产物L-缬氨酸浓度95 mmol/L. 该固定化细胞连续10次使用, 平均转化率为90.8%(以N-乙酰-L-缬氨酸计), 显示出了良好的工业化应用前景.

关键词: 乙酰鸟氨酸脱乙酰酶; 拆分; 固定化细胞; D,L-缬氨酸

Abstract:

D-Amino acids are widely used intermediates in pharmacy. D-Amino acids can be produced by chiral separation of D,L-amino acids racemate. The stereospecific hydrolysis of N-acetyl-D,L-amino acids by microbial enzymys is one of the most extensively used procedures in optical resolution of D,L-amino acids. In this report, a new method of chiral resolution of D,L-valine by immobilized cells with acetylornithine deacetylase activity was developed. The optimum reaction conditions were 200 mmol/L N-acetyl-D,L-valine and 0.2 g/mL(or 100 U/mL) of immobilized cells with 0.1 mmol/L CoCl2 as activator at pH=6. After 2—3 h incubation at 50 ℃, 95 mmol/L L-valine was obtained. In continuous use of immobilized cells for 10 times, the average conversion rate against N-acetyl-L-valine was 90.8%. The results display great potential in industrialization. This is the first report of chiral resolution of D,L-valine by acetylornithine deacetylase(ArgE).

Key words: Acetylornithine deacetylase; Chiral resolution; Immobilized cell; D,L-Aline

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