高等学校化学学报

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (5): 899.

• 研究论文 • 上一篇    下一篇

基于阳离子荧光共轭聚合物和核酸适体探针的蛋白质检测新方法

羊小海, 王胜锋, 王柯敏, 罗晓明, 谭蔚泓, 崔亮   

  1. 湖南大学化学生物传感与计量学国家重点实验室, 化学化工学院, 生物医学工程中心, 生物纳米与分子工程湖南省重点实验室, 长沙 410082
  • 收稿日期:2008-06-06 出版日期:2009-05-10 发布日期:2009-05-10
  • 通讯作者: 王柯敏, 男, 博士, 教授, 博士生导师, 从事纳米及分子水平上的生物分析化学及纳米生物技术研究, E-mail: kmwang@hnu.cn
  • 基金资助:

    国家“九七三”项目(批准号: 2002CB513110)、科技部国际合作重点项目(批准号: 2003DF000039)、国家自然科学基金(批准号: 90606003)和湖南省杰出青年科学基金(批准号: 08JJ1002)资助.

Novel Method of Protein Detection Based on Cationic Conjugated Polymer and Aptamer Probe

YANG Xiao-Hai, WANG Sheng-Feng, WANG Ke-Min*, LUO Xiao-Ming, TAN Wei-Hong, CUI Liang   

  1. State Key of Laboratory of Chemo/Biosensing and Chemomertrics, College of Chemistry and Chemical Engineering, Biomedical Engineering Center, Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China
  • Received:2008-06-06 Online:2009-05-10 Published:2009-05-10
  • Contact: WANG Ke-Min, E-mail: kmwang@hnu.cn

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被引次数

19

摘要：

以核酸适体为识别分子, 阳离子荧光共轭聚合物为报告分子, 建立了一种蛋白质检测新方法. 修饰有荧光熄灭基团的核酸适体探针通过静电作用与阳离子荧光共轭聚合物结合, 导致后者荧光熄灭. 当加入靶蛋白后, 核酸适体探针与其特异性结合, 荧光熄灭基团与阳离子荧光共轭聚合物远离, 聚合物荧光信号得以恢复. 实验结果表明, 荧光恢复程度与靶蛋白的浓度正相关. 采用该方法检测凝血酶的线性范围为17～40 nmol/L.

关键词: 核酸适体; 凝血酶; 阳离子共轭聚合物; 荧光检测

Abstract:

A novel fluorescence-based method for protein detection was developed based on aptamer probe as the recognized molecule and cationic conjugated polymers(CCP) as the reporter. The quencher-labeled aptamer probe attached to CCP by electrostatic interaction, leading to fluorescence quenching of CCP. When target protein was added, it bonded specifically to aptamer probe, which made the quencher detached from CCP. Then the fluorescence of CCP was resumed. The results show that the fluorescence resuming ratio is proportional to the concentration of target protein. The detection method for thrombin had a linear range of 17—40 nmol/L.

Key words: Aptamer; Thrombin; Cationic conjugated polymers; Fluorescence detection

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