硅表面CS/BSA复合微图形的制备及表征

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (11): 2319.

硅表面CS/BSA复合微图形的制备及表征

谢佳¹, 鲁雄^2,3, 张红平², 周先礼¹, 屈树新², 冯波², 翁杰²

1. 西南交通大学生命科学与工程学院,
2. 材料科学与工程学院, 材料先进技术教育部重点实验室, 成都 610031;
3. 香港科技大学机械工程系, 香港九龙清水湾

收稿日期:2009-01-16 出版日期:2009-11-10 发布日期:2009-11-10
通讯作者: 鲁雄, 男, 博士, 副教授, 主要从事生物材料及微加工方面的研究. E-mail: luxiong_2004@163.com
基金资助:
国家自然科学基金(批准号: 30700172)、自然科学基金中港联合基金(批准号: N_HKUST601/08, 30831160509)、高等学校博士学科点专项科研(新教师)基金(批准号: 20070613019)和国家科技支撑计划(批准号: 2006BAI16B01)资助.

Preparation and Characterization of the CS/BSA Composite Micropatterns on Silicon Surfaces

XIE Jia¹, LU Xiong^2,3*, ZHANG Hong-Ping², ZHOU Xian-Li¹, QU Shu-Xin², FENG Bo², WENG Jie²

1. School of Life Science and Engineering,
2. Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China;
3. Department of Mechanical Engineering, Hong Kong University of Science and Technology, HongKong, China

Received:2009-01-16 Online:2009-11-10 Published:2009-11-10
Contact: LU Xiong. E-mail: luxiong_2004@163.com
Supported by:
国家自然科学基金(批准号: 30700172)、自然科学基金中港联合基金(批准号: N_HKUST601/08, 30831160509)、高等学校博士学科点专项科研(新教师)基金(批准号: 20070613019)和国家科技支撑计划(批准号: 2006BAI16B01)资助.

摘要/Abstract

摘要：

采用微加工方法在硅表面成功地制备出壳聚糖/牛血清白蛋白(CS/BSA)复合微图形. 借助光镜和荧光显微镜对复合微图形进行形貌分析. 利用大肠杆菌和白色葡萄球菌定量考察了CS/BSA复合微图形的抗菌性能, 结果表明, 硅表面沟槽交叉状CS/BSA复合微图形对两种细菌都具有较好的抗菌效果. 通过MC3T3-E成骨细胞培养考察了复合微图形的细胞相容性, 结果表明, CS/BSA复合微图形对于细胞的生长方向具有较强的诱导性, 可促进细胞在材料表面的黏附、铺展及增殖分化. 结果表明, 采用微转移模塑法制备的CS/BSA复合微图形具有较好的抗菌性和细胞相容性.

关键词: 微图形; CS/BSA复合微图形; 微转移模塑法; 抗菌性; 细胞相容性

Abstract:

Chitosan/Bovine serum albumin(CS/BSA) composite micropatterns were successfully fabricated on silicon surfaces by micro-transfer molding method. The micropatterned CS/BSA composite films were stu-died by optical microscopy and fluorescent microscopy. A quantitative test was carried out to characterize the antibacterial properties of the CS/BSA composite films. The results show that the micropatterned CS/BSA composite films are effectively against E. coli and Staphylococcus albus. MC3T3-E cell culture test indicated that micropatterned CS/BSA composite films could strongly direct the orientation of cells. Moreover, the micropatterned CS/BSA composite films could promote the cell adhesion, spreading, proliferation and differen-tiation. Thus, the micropatterned CS/BSA composite film fabricated by micro-transfer molding method has good antibacterial properties and cell compatibility.

Key words: Micropattern; CS/BSA composite micropattern; Micro-transfer molding method; Antibacterial property; Cell compatibility

TrendMD:

谢佳, 鲁雄, 张红平, 周先礼, 屈树新, 冯波, 翁杰. 硅表面CS/BSA复合微图形的制备及表征. 高等学校化学学报, 2009, 30(11): 2319.

XIE Jia, LU Xiong*, ZHANG Hong-Ping, ZHOU Xian-Li, QU Shu-Xin, FENG Bo, WENG Jie. Preparation and Characterization of the CS/BSA Composite Micropatterns on Silicon Surfaces. Chem. J. Chinese Universities, 2009, 30(11): 2319.

参考文献

[1]JIANG Jia-Huan(蒋稼欢). Biomedical Microsystems Technology and Application(生物医学微系统技术及应用)[M], Beijing: Chemical Industry Press, 2006: 80—81
[2]Xia You-nan, Whitesides G. M.. Annu. Rev. Mater. Sci.[J], 1998, 28: 153—184
[3]Xia You-nan, Whitesides G. M.. Angew. Chem. Int. Ed.[J], 1998, 37: 550—575
[4]Michel R., Reviakine I., Sutherland D., et al.. Langmuir[J], 2002, 18(22): 8580—8586
[5]Falconne D., Pasqui D., Park S., et al.. Nano Lett.[J], 2004, 4(10): 1909—1914
[6]Masuda Y., Sugiyama T., Lin H., et al.. Thin Solid Films[J], 2001, 382(1/2): 153—157
[7]Michel R., Lussi J. W., Csucs G., et al.. Langmuir[J], 2002, 18(8): 3281—3287
[8]Ravi Kumar M. N. V.. Reactive and Functional Polymers[J], 2000, 46(1): 1—27
[9]Sorlier P., Denuziere A., Viton C., et al.. Biomacromolecules[J], 2001, 2(3): 765—772
[10]Constantine C. A., Mello S. V., Dupont A., et al.. J. Am.Chem. Soc.[J], 2003, 125(7): 1805—1809
[11]Camilo C. S., Santos D. S., Rodrigues J. J., et al.. Biomacromolecules[J], 2003, 4(6): 1583—1588
[12]FU Yin(付寅), YAO Gui-Jun(姚桂君), DONG Yu-Ping(董宇平), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2003, 24(1): 181—183
[13]FENG Jie, GAO Chang-you, WANG Bo, et al.. Thin Solid Films[J], 2004: 286—290
[14]Franssila S.. Introduction to Microfabrication(微加工导论)[M], Beijing: Electronics Industry Press, 2006: 154—163
[15]HU Ying(胡瑛), DU Yu-Min(杜予民), LIU Hui(刘慧). J. Analytical Science(分析科学学报)[J], 2003, 19(4): 305—308
[16]ZHANG Jun-Ying(张俊英), GE Qi(葛琦), YANG Chun(杨春), et al.. Rare Metal Materials and Engineering(稀有金属材料与工程)[J], 2007, 36(6): 1037—1040
[17]SHEN Yu-Hua(沈玉华), YANG Zhan-Lan(杨展澜), WU Jin-Guang(吴瑾光). Acta Scientiarum Naturalium Universitatis Pekinensis(北京大学学报, 自然科学版)[J], 1999, 35(4): 431—436
[18]BAO Zhi-Ming(包志明), PAN Ji-Lun(潘继伦), WU Chen(吴晨), et al.. J. Functional Materials(功能材料)[J], 2004, 35: 2454—2456
[19]ZHANG Li(张莉), ZHUO Xin(卓馨), WANG Hong-Yan(王红艳), et al.. J. Inorganic Chemistry(无机化学学报)[J], 2007, 23(11): 1988—1993
[20]Fukuzaki S., Urano H., Nagata K.. J. Fermentation Bioengineering[J], 1996, 81(2): 163—167
[21]Kopac T., Bozgeyik K., Yener J.. Colloids and Surfaces A: Physicochem. Eng. Aspects[J], 2008, 322: 19—28
[22]Kurrat R., Prenosil J. E., Ramsden J. J.. J. Colloid Interface Sci.[J], 1997, 185: 1—8
[23]CHEN Yu(陈瑜), WU Jun-Xu(吴俊徐), GUO Ping-Sheng(郭平生), et al.. Microfabrication Technology(微细加工技术)[J], 2005, 4: 37—41
[24]Dutta P. K., Tripathi S., Mehrotra G. K., et al.. Food Chemistry[J], 2009, 114: 1173—1182
[25]Seiichi T., Yoshiaki M., Masayoshi J., et al.. J. Controlled Release[J], 1994, 28(1—3): 235—241
[26]Krsko P., McCann T. E., Thach T. T.. Biomaterials[J], 2009, 30(5): 721—729
[27]Recknor J. B., Recknor J. C., Sakaguchi D. S., et al.. Biomaterials[J], 2004, 25(14): 2753—2767
[28]WU Feng(吴峰). Chitosan Complexed Films Investigated by Atomic Force Microscopy(壳聚糖复合膜的制备及表面形貌的原子力显微镜观察)[D], Guangzhou: University of Jinan, 2006
[29]HOU Yue(侯悦), LIN Quan-Kui(林全愧), JI Jian(计剑), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2008, 29(9): 1890—1894