裙带菜中聚甘露糖醛酸的分离纯化与结构分析

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (11): 2189.

裙带菜中聚甘露糖醛酸的分离纯化与结构分析

王彩¹, 施松善¹, 洪其明², 王顺春¹, 王峥涛¹, 胡之璧¹

1. 上海中医药大学中药研究所, 教育部中药标准化研究重点实验室,
2. 中药学院, 上海 201210

收稿日期:2009-03-19 出版日期:2009-11-10 发布日期:2009-11-10
通讯作者: 王顺春, 男, 博士, 研究员, 博士生导师, 主要从事糖化学和糖生物学研究. E-mail: shunchunwang@126.com
基金资助:
国家“八六三”计划(批准号: 2006AA090405)和上海市曙光计划项目(批准号: 08SG44)资助.

Isolation, Purification and Structural Analysis of Polymannuronic Acid from Undaria pinnatifida

WANG Cai¹, SHI Song-Shan¹, HONG Qi-Ming², WANG Shun-Chun^1*, WANG Zheng-Tao¹, HU Zhi-Bi¹

1. Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica,
2. College of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China

Received:2009-03-19 Online:2009-11-10 Published:2009-11-10
Contact: WANG Shun-Chun. E-mail: shunchunwang@126.com
Supported by:
国家“八六三”计划(批准号: 2006AA090405)和上海市曙光计划项目(批准号: 08SG44)资助.

摘要/Abstract

摘要：

以裙带菜(Undaria pinnatifida, wakame)为原料, 经水提醇沉、DEAE-Sepharose Fast Flow、Sephacryl S-300和Sephacryl S-200凝胶柱分离纯化, 得到2个酸性多糖UPPS03和UPPS04. 高效凝胶渗透色谱测试结果表明, 其为均一多糖, 平均分子量分别为3.6×10⁴和1.1×10⁴. 采用糖组成分析、高碘酸氧化及Smith降解、糖醛酸还原、甲基化、红外光谱和核磁共振等方法对该多糖的化学结构进行了表征. 结果表明, 2个多糖均为1,4连接的聚甘露糖醛酸.

关键词: 裙带菜; 聚甘露糖醛酸; 分离纯化; 结构分析

Abstract:

UPPS03 and UPPS04 were obtained from Undaria pinnatifida by water extraction, DEAE-Sepharose Fast Flow, Sephacryl S-300 and Sephacryl S-200 gel permeation chromatography. HPGPC shows that both of them are homogeneous polysaccharides with an average molecular weigh of 3.6×10⁴ and 1.1×10⁴, respectively. The structural features of UPPS03 and UPPS04 were characterized with sugar composition analysis, periodic acid oxidation and Smith degradation, reduction of uronic acid, methylation analysis, IR and NMR. The results indicate that they are all linear polysaccharide with β-1,4-linked polymannuronic acid, and this is the first report of polymannuronic acid isolated from Undaria pinnatifida.

Key words: Undaria pinnatifida; Polymannuronic acid; Isolation and purification; Structural analysis

TrendMD:

王彩, 施松善, 洪其明, 王顺春, 王峥涛, 胡之璧. 裙带菜中聚甘露糖醛酸的分离纯化与结构分析. 高等学校化学学报, 2009, 30(11): 2189.

WANG Cai, SHI Song-Shan, HONG Qi-Ming, WANG Shun-Chun*, WANG Zheng-Tao, ....... Isolation, Purification and Structural Analysis of Polymannuronic Acid from Undaria pinnatifida. Chem. J. Chinese Universities, 2009, 30(11): 2189.

参考文献

[1]Hemmingson J. A., Falshaw R., Furneaux R. H.. et al.. Appl. Phycol.[J], 2006, 18: 185—193
[2]Maruyama H., Tamauchi H., Hashimoto M., et al.. In Vivo[J], 2003, 17: 245—250
[3]Maruyama H., Tamauchi H., Hashimoto M., et al.. Int. Arch. Allergy Immunol.[J], 2005, 137: 289—294
[4]LAI Xiao-Fang(赖晓芳), SHEN Shan-Rui(沈善瑞), LI Jie(李杰). Bulletin of Science and Technology(科技通报)[J], 2007, 23(4): 483—487
[5]SUN Jing-Ya(孙静亚), WANG Hui-Jun(王慧君). Chin. J. Mar. Drugs(中国海洋药物)[J], 2008, 27(5): 46—51
[6]KANG Yan-Yan(康琰琰), WANG Yi-Fei(王一飞), ZHU Liang(朱良), et al.. J. Chin. Med. Mat.(中药材)[J], 2005, 28(9): 769—771
[7]KANG Yan-Yan(康琰琰), WANG Yi-Fei(王一飞), XIONG Sheng(熊盛), et al.. Chin. Pharm. J.(中国药学杂志)[J], 2006, 41(22): 1748—1750
[8]WU Yuan-Feng(吴元锋), LI Ya-Fei(李亚飞), LIU Shi-Wang(刘士旺), et al.. Transactions of the CSAE(农业工程学报)[J], 2008, 24(6): 273—276
[9]WEI Yuan-An(魏远安), FANG Ji-Nian(方积年). Acta Pharmaceutica Sinica(药学学报)[J], 1989, 24(7): 532—536
[10]Stevenson T. T., Furneaux R. H.. Carbohydrate Research[J], 1991, 210: 277—298
[11]ZHANG Wei-Jie(张惟杰). Biochemical Research Technology of Glycoconjugate, 2nd Ed.(糖复合物生化研究技术, 第二版)[M], Hangzhou: Zhejiang University Press, 1999: 140—142
[12]Senchenkova S. N., Knirel Y. A., Likhosherstov L. M., et al.. Carbohydrate Research[J], 1995, 266(1): 103—113
[13]Needs P. W., Selvendran R. R.. Carbohydrate Research[J], 1993, 245: 1—10
[14]Blakeney A. B., Harris P. J., Henry R. J., et al.. Carbohydrate Research[J], 1983, 113: 291—299
[15]Leal D., Matsuhiro B., Rossi M., et al.. Carbohydrate Research[J], 2008, 343: 308—316
[16]ZHANG Hong-Rong(张洪荣), WANG Chang-Yun(王长云), LIU Bin(刘斌), et al.. Chin. J. Mar. Drugs(中国海洋药物杂志)[J], 2006, 25(3): 1—6
[17]LIU Bin(刘斌), WANG Chang-Yun(王长云), ZHANG Hong-Rong(张洪荣), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2006, 27(3): 485—487
[18]Grasdalen H., Larsen B., Smidsrod A. O.. Carbohydrate Research[J], 1981, 89: 179—191
[19]Chhatbar M., Meena R., Prasad K., et al.. Carbohydrate Polymers[J], 2009, 76: 650—656
[20]XU Xu(续旭), YU Guang-Li(于广利), LIU Bin(刘斌), et al.. Journal of Ocean University of China(中国海洋大学学报)[J], 2004, 34(2): 2l7—223
[21]Gacesa P.. Carbohydrate Polymers[J], 1988, 8: 161—182