基于金胶的选择性聚集实现p53基因的电化学超灵敏检测

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (11): 2170.

基于金胶的选择性聚集实现p53基因的电化学超灵敏检测

郑静^1,2, 沈碧君¹, 程圭芳¹, 冯婉娟¹, 何品刚¹, 方禹之¹

1. 华东师范大学化学系, 上海 200062;
2. 上海工程技术大学化学化工学院, 上海 201620

收稿日期:2009-03-12 出版日期:2009-11-10 发布日期:2009-11-10
通讯作者: 程圭芳, 女, 博士, 副教授, 主要从事生物传感器、电化学及纳米材料等方面的研究. E-mail: gfcheng@chem.ecnu.edu.cn
基金资助:
国家自然科学基金(批准号: 20675031)、上海市科委浦江人才计划(批准号: 06PJ14032)和上海工程技术大学校发展基金(批准号: 2008xy32)资助.

Ultrasensitive Electrochemical Assay for the Detection of p53 Sequence Based on the Aggregation of Gold Nanoparticles

ZHENG Jing^1,2, SHEN Bi-Jun¹, CHENG Gui-Fang^1*, FENG Wan-Juan¹, HE Pin-Gang¹, FANG Yu-Zhi¹

1. Department of Chemistry, East China Normal University, Shanghai 200062, China;
2. Department of Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China

Received:2009-03-12 Online:2009-11-10 Published:2009-11-10
Contact: CHENG Gui-Fang. E-mail: gfcheng@chem.ecnu.edu.cn
Supported by:
国家自然科学基金(批准号: 20675031)、上海市科委浦江人才计划(批准号: 06PJ14032)和上海工程技术大学校发展基金(批准号: 2008xy32)资助.

摘要/Abstract

摘要：

介绍了一种利用金胶的选择性聚集实现信号扩增的超灵敏的电化学方法, 用于人类p53肿瘤抑制剂基因的检测. 在实验中, 根据p53基因的序列设计了能特异性检测p53肿瘤抑制剂基因的二段探针, 在一段探针上固定磁性颗粒以捕获并富集目标基因, 同时在另一段探针上标记金纳米颗粒作为检测信标. 另外, 通过硫代三聚氰酸和金纳米颗粒的自组装作用, 形成金纳米颗粒和硫代三聚氰酸的网状结构, 获得金纳米颗粒的选择性聚集, 实现信号扩增. 用此法检测目标p53野生型DNA, 最低检测限为2.24×10^-17 mol/L, 同时进一步研究了该探针对p53野生型和一碱基错配的突变型的选择性.

关键词: 金纳米颗粒; p53基因; 电化学检测

Abstract:

An ultrasensitive and high specific electrochemical assay for the detection of human tumor suppression p53 gene has been developed. Two DNA sequences were designed, and the combined sequences were complementary to that of the target p53 sequence, one was immobilized on the magnetic nanoparticles for capturing the target p53 sequence, the other was labeled with gold nanoparticles as probe. The ultrasensitive detection was achieved by the aggregation of gold nanoparticles with introducing thiocyanuric acid into the system to form the network like structure, and the detection limit could go down to 2.24×10^-17 mol/L for the wild type p53 sequence. The discrimination between the mutant type p53 DNA and the wide type p53 DNA was studied with the proposed assay.

Key words: Gold nanoparticle; p53 Gene; Electrochemical detection

TrendMD:

郑静, 沈碧君, 程圭芳, 冯婉娟, 何品刚, 方禹之. 基于金胶的选择性聚集实现p53基因的电化学超灵敏检测. 高等学校化学学报, 2009, 30(11): 2170.

ZHENG Jing, SHEN Bi-Jun, CHENG Gui-Fang*, FENG Wan-Juan, HE Pin-Gang, ....... Ultrasensitive Electrochemical Assay for the Detection of p53 Sequence Based on the Aggregation of Gold Nanoparticles. Chem. J. Chinese Universities, 2009, 30(11): 2170.

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