高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

键合紫杉醇的纳米胶束与C6胶质瘤细胞的相互作用

王占峰1, 罗毅男1, 郑勇辉2, 韩海玲3, 洪新雨1, 景遐斌2   

    1. 吉林大学第一临床医院神经外科, 长春 130021;
    2. 中国科学院长春应用化学研究所高分子物理与化学国家重点实验室, 长春 130022;
    3. 北华大学附属医院心脑科, 吉林 132011
  • 收稿日期:2007-12-18 修回日期:1900-01-01 出版日期:2008-08-10 发布日期:2008-08-10
  • 通讯作者: 景遐斌

Interaction of Copolymer-paclitexal Conjugate Micelles with C6 Glioma Cells

WANG Zhan-Feng1, LUO Yi-Nan1, ZHENG Yong-Hui2, HAN Hai-Ling3, HONG Xin-Yu1, JING Xia-Bin2*   

    1. Department of Neurosurgery, First Clinical Hospital, Jilin University, Changchun 130021, China;
    2. State Key Lab of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    3. Department of Cardiac, Affiliated Hospital of Beihua University, Jilin 132011, China
  • Received:2007-12-18 Revised:1900-01-01 Online:2008-08-10 Published:2008-08-10
  • Contact: JING Xia-Bin

PDF (PC)

摘要： 制备了键合紫杉醇(PTX)的聚乙二醇-聚乳酸嵌段共聚物(PEG-PLA/PTX)的纳米胶束, 采用四氮唑(MTT)比色法、流式细胞术、透射电镜及激光共聚焦显微镜等技术, 考察了PEG-PLA/PTX胶束对C6胶质瘤细胞的影响, 包括C6细胞超微结构的变化和细胞周期的改变, 以及纳米颗粒在细胞内的分布, 探讨了PEG-PLA/PTX胶束对肿瘤细胞的作用机理. 结果表明, PEG-PLA/PTX胶束进入到C6细胞内, 聚集于细胞浆中, 通过与细胞核中DNA的作用改变细胞生长的周期, 造成在G2-M期的阻滞, 引起细胞的凋亡. 因此, PEG-PLA/PTX胶束有望用于脑胶质瘤的化疗.

关键词: 键合紫杉醇, 聚乙二醇-聚乳酸嵌段共聚物, 流式细胞术, 透射电镜, 激光共聚焦显微镜, C6胶质瘤细胞, 诱导凋亡, 细胞周期.

Abstract: Nanoscale micelles of poly(ethylene)-b-poly(lactic acid)/paclitexal conjugate(PEG-PLA/PTX) were prepared and their interaction with rat brain glioma C6 cells was examined by MTT, electron microscopy, flow cytometry(FCM), and confocal laser scanning microscopy(CLSM). The results show that the copolymer/paclitaxel conjugate micelles can enter the cell, and are distributed in cytoplasm. The paclitaxel can be released from the conjugate micelles and react with the genes in the cell nuclei, causing changes in the gene molecules, and thus, leading to changes in growth cycle of the cells, resulting in G2-M cell cycle arrest and inducing apoptosis of C6 cells. Therefore, PEG-PLA/PTX micelles may be used in chemotherapy of brain glioma.

Key words: Paclitaxel conjugate, Poly(ethylene)-b-poly(lactic acid) block copolymer, Flow cytometry, TEM, CLSM, Glioma C6 cells, Apoptosis, Cell cycle

中图分类号: 

TrendMD: