高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

不同介质中蜂毒素聚集/解聚集的超分子调控及相关机理

王磊1, 郝雅琼1, 李岳1, 李正强3, 吴玉清1,2   

    1. 吉林大学超分子结构与材料国家重点实验室, 长春 130012;
    2. 复旦大学聚合物分子工程教育部重点实验室, 上海 200433;
    3. 吉林大学分子酶学工程教育部重点实验室, 长春130021
  • 收稿日期:2007-10-09 修回日期:1900-01-01 出版日期:2008-06-10 发布日期:2008-06-10
  • 通讯作者: 吴玉清

Supramolecular Monitoring and the Related Mechanism of Aggregation/Disaggregation of Melittin in Different Media

WANG Lei1, HAO Ya-Qiong1, LI Yue1, LI Zheng-Qiang3, WU Yu-Qing1,2*   

    1. State Key Laboratory for Supramolecular Structure and Materials, Jilin University, Changchun 130012, China;
    2. Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Fudan University, Shanghai 200433, China;
    3. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130021, China
  • Received:2007-10-09 Revised:1900-01-01 Online:2008-06-10 Published:2008-06-10
  • Contact: WU Yu-Qing

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被引次数

1

摘要： 利用荧光光谱和圆二色谱等技术手段研究了蜂毒素在2-羟丙基环糊精、氯化钠或 DOPC 调控下的聚集/解聚集过程、相关机理以及不同介质中α-helix的含量. 研究结果表明, 蜂毒素在不同介质中的聚集能力、构象以及和介质分子相互作用力均存在很大差别.

关键词: 蜂毒素, 2-羟丙基环糊精, 氯化钠, DOPC, α-螺旋

Abstract: Melittin, with strong pharmacological effects and biological activity, is the major component of bee venom. The physiological function of melittin depends upon its secondary structure and aggregation state due to which the peptide loses its activity. Once the aggregation/disaggregation processes can be effectively monitored, it will lay the foundation for clinical applications by exploring its function and mechanism. In this paper the aggregation/disaggregation processes were explored, the related mechanism and the α-helix content of melittin in aqueous solution is controlled by 2-hydroxypropyl-β-cyclodextrin, sodium chloride or 1,2-dioleoyl-sn-glycero-3-phosphocholine(DOPC) through fluorescence spectra and circular dichroism(CD).

Key words: Melittin, 2-Hydroxypropyl-β-cyclodextrin, NaCl, 1,2-Dioleoyl-sn-glycero-3-phosphocholine(DOPC), α-Helix

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