结合丝组二肽的树状多肽对λDNA的切割

高等学校化学学报

结合丝组二肽的树状多肽对λDNA的切割

何骏, 麻远, 赵玉芬

清华大学化学系, 生命有机磷化学及化学生物学教育部重点实验室, 北京 100084

收稿日期:2005-10-11 修回日期:1900-01-01 出版日期:2006-10-10 发布日期:2006-10-10
通讯作者: 麻远

Cleavage of λDNA by Seryl-histidine Planted Peptide Dendrimer

HE Jun, MA Yuan, ZHAO Yu-Fen

The Key laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China

Received:2005-10-11 Revised:1900-01-01 Online:2006-10-10 Published:2006-10-10
Contact: MA Yuan

摘要/Abstract

摘要： 以Fmoc手工固相合成法合成了以多聚赖氨酸为骨架, 表面结合丝组二肽的四分支和二分支树状多肽, 以高效液相色谱提纯, 电喷雾电离质谱表征, 并通过凝胶电泳法研究了其对λDNA的切割活性.

关键词: 丝组二肽, 树状多肽, DNA切割

Abstract: Artificial nucleic acid cleavage agents have received considerable attention due to their very important applications in biochemistry and molecular biology. Seryl-histidine is a dipeptide possessing DNA, protein and ester cleavage activity. Since peptide dendrimers have a lot of unique physical and chemical properties compared to normal peptide chain and have a great varieties of applications in biological areas, two and four branched seryl-histidine planted peptide dendrimers were synthesized by Fmoc solid phase peptide synthesis approach. The products were purified by HPLC and characterized by ESI-MS. Their DNA cleavage activities were tested by means of Agarose Gel Electrophoresis. In the cleavage reaction, peptide dendrimer showed a strong combining inclination to DNA, highly concentrated dendrimer solution can quench the fluorescence of EB. Even though, the DNA cleavage activity of the dendrimers was lower than that of seryl-histidine dipeptide monomer.

Key words: Seryl-histdine dipeptide, Peptide dendrimer, DNA cleavage

中图分类号:

O629.72

TrendMD:

何骏, 麻远, 赵玉芬 . 结合丝组二肽的树状多肽对λDNA的切割. 高等学校化学学报, doi: .

HE Jun, MA Yuan, ZHAO Yu-Fen. Cleavage of λDNA by Seryl-histidine Planted Peptide Dendrimer. Chem. J. Chinese Universities, doi: .

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