高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 97.

• Analytical Sciences • 上一篇    下一篇

Rapid Determination of Organophosphorus and Carbamate Pesticide Residues in Vegetables and Fruits Using a Plant-Hydrolases Inhibition Technique

ZHONG Shu-Ming, YUAN Dong-Xing, XU Peng-Xiang   

  1. Environmental Science Research Center, Xiamen University, Xiamen 361005
  • 出版日期:2000-12-31 发布日期:2000-12-31

Rapid Determination of Organophosphorus and Carbamate Pesticide Residues in Vegetables and Fruits Using a Plant-Hydrolases Inhibition Technique

ZHONG Shu-Ming, YUAN Dong-Xing, XU Peng-Xiang   

  1. Environmental Science Research Center, Xiamen University, Xiamen 361005
  • Online:2000-12-31 Published:2000-12-31

PDF (PC)

摘要：

The wide use of toxic organophosphorus and carbamates pesticides in agriculture and the concern of its effects on die environment have called for the need of sensitive and portable detection methods in vegetable and fruit survey. The plant hydrolases can catalyze the hydrolysis of substrate 2,6-dichlorophenolindophenol acetate, changing its colour from yellow to deep blue in aqueous solution. When the plant hydrolases are in the presence of the pesticides, the enzyme activity decreases, resulting in the change of colour.

Abstract:

The wide use of toxic organophosphorus and carbamates pesticides in agriculture and the concern of its effects on die environment have called for the need of sensitive and portable detection methods in vegetable and fruit survey. The plant hydrolases can catalyze the hydrolysis of substrate 2,6-dichlorophenolindophenol acetate, changing its colour from yellow to deep blue in aqueous solution. When the plant hydrolases are in the presence of the pesticides, the enzyme activity decreases, resulting in the change of colour.

TrendMD: