高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 88.

• Analytical Sciences • 上一篇    下一篇

Determination of Proteins at Nanogram Levels by Enhanced Rayleigh Light Scattering Technique with Tetra-Substituted Sulphonated Aluminum Phthalocyanine

CHEN Xiao-Lan, LI Dong-Hui, ZHU Qing-Zhi, YANG Huang-Hao, XU Jin-Gou   

  1. Department of Chemistry, the Key Laboratory of Analytical Science of MOE, Xiamen University, Xiamen 361005
  • 出版日期:2000-12-31 发布日期:2000-12-31
  • 通讯作者: XU Jin-Gou

Determination of Proteins at Nanogram Levels by Enhanced Rayleigh Light Scattering Technique with Tetra-Substituted Sulphonated Aluminum Phthalocyanine

CHEN Xiao-Lan, LI Dong-Hui, ZHU Qing-Zhi, YANG Huang-Hao, XU Jin-Gou   

  1. Department of Chemistry, the Key Laboratory of Analytical Science of MOE, Xiamen University, Xiamen 361005
  • Online:2000-12-31 Published:2000-12-31
  • Contact: XU Jin-Gou

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摘要：

In recent years, Rayleigh light scattering has become a new tool for determining the content of biological molecules and studying the interaction mechanism of organic dyes with biological molecules[1]. According to the macroscopic fluctuation theory, in a transparent isotropic medium, when the light scattering is caused by molecular particles 20-fold smaller than the wavelength of the incident beam, the Rayleigh scattering law is obeyed, namely I∝1/λ4[2]. However, if the wavelength of the incident beam is close to that of the absorption band of the molecular particles which exist as aggregates, Rayleigh scattering will deviate from the law and enhanced RLS can be expected[3]. Using this technique, a method for the determination of proteins in aqueous solution has been developed based on the enhancement effect of proteins on die Rayleigh light scattering (RLS) of organic dyes[4].

Abstract:

In recent years, Rayleigh light scattering has become a new tool for determining the content of biological molecules and studying the interaction mechanism of organic dyes with biological molecules[1]. According to the macroscopic fluctuation theory, in a transparent isotropic medium, when the light scattering is caused by molecular particles 20-fold smaller than the wavelength of the incident beam, the Rayleigh scattering law is obeyed, namely I∝1/λ4[2]. However, if the wavelength of the incident beam is close to that of the absorption band of the molecular particles which exist as aggregates, Rayleigh scattering will deviate from the law and enhanced RLS can be expected[3]. Using this technique, a method for the determination of proteins in aqueous solution has been developed based on the enhancement effect of proteins on die Rayleigh light scattering (RLS) of organic dyes[4].

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