高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 87.

• Analytical Sciences • 上一篇    下一篇

Determination of Trace Quinhydrone by Enzymatic Kinetic Spectrophotometry

LIN Xin-Hua, LI Chun-Yan   

  1. Department of Chemistry, Fujian Medical University, Fuzhou 350004
  • 出版日期:2000-12-31 发布日期:2000-12-31

Determination of Trace Quinhydrone by Enzymatic Kinetic Spectrophotometry

LIN Xin-Hua, LI Chun-Yan   

  1. Department of Chemistry, Fujian Medical University, Fuzhou 350004
  • Online:2000-12-31 Published:2000-12-31

摘要:

Only trace of quinhydrone, a urease-inhibitor, can inhibit enzymatically promoting hydrolytic reaction of urea[1]. A new enzymatic inhibition kinetic spectrophotometry method[2] for determination of trace quinhydrone was obtained by urea and P-dimethylamino-benzadehyde (color reagent) developing action. In this reaction, maximal absorptive wavelength is 420 nm. The enzymatic promoting reaction rate, log (A0/A1),enzymatic inhibition reaction rate, log (A0/A2,and their difference, log (A2/A1), are measured by detecting the remains of urea. All factors (urease, urea and color reagent dosage; reaction temperature; heating time), effecting log (A2/A1) were investigated.

Abstract:

Only trace of quinhydrone, a urease-inhibitor, can inhibit enzymatically promoting hydrolytic reaction of urea[1]. A new enzymatic inhibition kinetic spectrophotometry method[2] for determination of trace quinhydrone was obtained by urea and P-dimethylamino-benzadehyde (color reagent) developing action. In this reaction, maximal absorptive wavelength is 420 nm. The enzymatic promoting reaction rate, log (A0/A1),enzymatic inhibition reaction rate, log (A0/A2,and their difference, log (A2/A1), are measured by detecting the remains of urea. All factors (urease, urea and color reagent dosage; reaction temperature; heating time), effecting log (A2/A1) were investigated.

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