高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 74.

• Analytical Sciences • 上一篇    下一篇

Study of the Electrochemiluminescence Based on the Reaction of p-Hydroxyphenylpyruvic Acid with Tris(2,2'-bipyridine)ruthenium(Ⅱ)

CHI Yu-Wu1, DUAN Jian-Ping2, CHEN Guo-Nan1   

  1. 1. Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002;
    2. The Central Laboratory, Fuzhou University, Fuzhou, Fujian 350002
  • 出版日期:2000-12-31 发布日期:2000-12-31
  • 基金资助:

    This study was supported by the National Nature Sciences Funding of China (29675003) and the Natural Sciences Foundation of Fujian Province, China (139810006).

Study of the Electrochemiluminescence Based on the Reaction of p-Hydroxyphenylpyruvic Acid with Tris(2,2'-bipyridine)ruthenium(Ⅱ)

CHI Yu-Wu1, DUAN Jian-Ping2, CHEN Guo-Nan1   

  1. 1. Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002;
    2. The Central Laboratory, Fuzhou University, Fuzhou, Fujian 350002
  • Online:2000-12-31 Published:2000-12-31
  • Supported by:

    This study was supported by the National Nature Sciences Funding of China (29675003) and the Natural Sciences Foundation of Fujian Province, China (139810006).

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摘要：

p-hydroxyphenylpyruvic acid (pHPP) is an important intermediate in the metabolism of tyrosine. Under normal circumstances pHPF is readily converted to 2,5-dihydroxyphenylacetic acid by p-hydroxy-phenylpyruvate oxidase and the levels of pHPP encountered in blood and urine are extremely low. However, in a few individuals suffering from a congenital metabolic defect known as tyrosinemia, the oxidase is not available which leads to dramatic elevation of pHPP levels in blood and urine[1]. Therefore, a selective and sensitive method for measuring pHPT is very useful for the diagnosis and treatment of such disease. In this paper the electrochemiluminescence(ECL) based on the reaction of pHPP with tris(2,2'-bipyridine)ruthenium(Ⅱ)[Ru(bpy)32+] in a flow injection system has been studied and a sensitive ECL method has been developed to detect pHPP in aqueous solution.

Abstract:

p-hydroxyphenylpyruvic acid (pHPP) is an important intermediate in the metabolism of tyrosine. Under normal circumstances pHPF is readily converted to 2,5-dihydroxyphenylacetic acid by p-hydroxy-phenylpyruvate oxidase and the levels of pHPP encountered in blood and urine are extremely low. However, in a few individuals suffering from a congenital metabolic defect known as tyrosinemia, the oxidase is not available which leads to dramatic elevation of pHPP levels in blood and urine[1]. Therefore, a selective and sensitive method for measuring pHPT is very useful for the diagnosis and treatment of such disease. In this paper the electrochemiluminescence(ECL) based on the reaction of pHPP with tris(2,2'-bipyridine)ruthenium(Ⅱ)[Ru(bpy)32+] in a flow injection system has been studied and a sensitive ECL method has been developed to detect pHPP in aqueous solution.

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