高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 70.

• Analytical Sciences • 上一篇    下一篇

Sensitive Liquid Chromatographic Determination of Neurotoxin β-ODAP and Non-protein Amino Acids in Lathyrus sativus by Precolumn Derivatization with Dansyl Chloride

WANG Fei1,2, XING Gen-Shen2, CHEN Xiong2, LI Zhi-Xiao1   

  1. 1. National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000;
    2. State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000
  • 出版日期:2000-12-31 发布日期:2000-12-31

Sensitive Liquid Chromatographic Determination of Neurotoxin β-ODAP and Non-protein Amino Acids in Lathyrus sativus by Precolumn Derivatization with Dansyl Chloride

WANG Fei1,2, XING Gen-Shen2, CHEN Xiong2, LI Zhi-Xiao1   

  1. 1. National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000;
    2. State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000
  • Online:2000-12-31 Published:2000-12-31

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摘要：

Lathyrus sativus (L. sativus) is a highly drought resistant and protein-rich leguminous crop cultivated in Africa and Asia, where it is a major protein source for people in the lowest income groups. However, excessive ingestion of this pulse can lead to irreversible paralysis of the legs-a disease known as neurolathyrism or lathyrism. The causative agent was reported to be the nonprotein amino acid, 3-N-L-oxalyl-2,3-diaminopropionic acid (β-ODAP)[1]. The α-isomer of ODAP has been shown to be nontoxic. L. sativus (shan li dou in China) shows good adaptation to the low rainfall conditions of northwestern China. Our group was exploring the breeding low or zero toxin varieties of L. sativus as grain crops for human consumption and as protein-rich feed for animals. Thus, it is necessary to develop a method to determine the toxin and other amino acids. Derivatization by 5-dimethylaminonaphthalene-l-sulfonyl chloride (DnsCl) as derivatization reagent was used for analysis of amino acids by Negro et al.[3] Sanz[4]. We have developed a HPLC method that can simultaneously determination the a- and|3-ODAP and other amino acids in L. sativus by dansylation. The method provides a simple accurate alternative to existing methods for plant screening purpose.

Abstract:

Lathyrus sativus (L. sativus) is a highly drought resistant and protein-rich leguminous crop cultivated in Africa and Asia, where it is a major protein source for people in the lowest income groups. However, excessive ingestion of this pulse can lead to irreversible paralysis of the legs-a disease known as neurolathyrism or lathyrism. The causative agent was reported to be the nonprotein amino acid, 3-N-L-oxalyl-2,3-diaminopropionic acid (β-ODAP)[1]. The α-isomer of ODAP has been shown to be nontoxic. L. sativus (shan li dou in China) shows good adaptation to the low rainfall conditions of northwestern China. Our group was exploring the breeding low or zero toxin varieties of L. sativus as grain crops for human consumption and as protein-rich feed for animals. Thus, it is necessary to develop a method to determine the toxin and other amino acids. Derivatization by 5-dimethylaminonaphthalene-l-sulfonyl chloride (DnsCl) as derivatization reagent was used for analysis of amino acids by Negro et al.[3] Sanz[4]. We have developed a HPLC method that can simultaneously determination the a- and|3-ODAP and other amino acids in L. sativus by dansylation. The method provides a simple accurate alternative to existing methods for plant screening purpose.

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