高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 340.

• Chemistry in Materials Sciences • 上一篇    下一篇

A Novel Application of Capillary Electrophoresis for Evaluating the Mutation Effects of Stevia Induced by Ion Implantation

HU Yong-Gang1, SHAO Han-Juan2, HU Rui1, CHEN Mu-Zhuan2   

  1. 1. National Lab. of Biomedical Photonics, Department of Environmental Science, Huazhong University of Science and Technology, Hubei 430074;
    2. The Key Lab. of the Minitry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005
  • 出版日期:2000-12-31 发布日期:2000-12-31
  • 基金资助:

    Supported by the National Natural Science Foundation of China, Chenguang Project of Wuhan and Visiting Scholar Foundation of Key Lab. in University.

A Novel Application of Capillary Electrophoresis for Evaluating the Mutation Effects of Stevia Induced by Ion Implantation

HU Yong-Gang1, SHAO Han-Juan2, HU Rui1, CHEN Mu-Zhuan2   

  1. 1. National Lab. of Biomedical Photonics, Department of Environmental Science, Huazhong University of Science and Technology, Hubei 430074;
    2. The Key Lab. of the Minitry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005
  • Online:2000-12-31 Published:2000-12-31
  • Supported by:

    Supported by the National Natural Science Foundation of China, Chenguang Project of Wuhan and Visiting Scholar Foundation of Key Lab. in University.

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摘要：

Stevia rebaudiana is a member of the Compositae, native to Paraguay. It produces a number of high-potency low-carlorie sweeteners in its leaf tissue. The sweeteners are diterpene glycosides and range between 30 and 320 times sweeter than sugar. Therefore, Stevia is likely to become a major source of high-potency sweetener for the growing natural food market in the future. Generally, techniques of ion implantation have been used to treat plant seeds and induce mutation for increasing the produce of stevia sweeteners. Various methods for analysis products of stevia rebaudiana have been reported, including gas-liquid chromatography, thin-layer chromatography and HPLC etc. However, these methods are time consuming. The separation and determination of stevia sweeteners by capillary electrophoresis was successfully demonstrated recently[1,2].

Abstract:

Stevia rebaudiana is a member of the Compositae, native to Paraguay. It produces a number of high-potency low-carlorie sweeteners in its leaf tissue. The sweeteners are diterpene glycosides and range between 30 and 320 times sweeter than sugar. Therefore, Stevia is likely to become a major source of high-potency sweetener for the growing natural food market in the future. Generally, techniques of ion implantation have been used to treat plant seeds and induce mutation for increasing the produce of stevia sweeteners. Various methods for analysis products of stevia rebaudiana have been reported, including gas-liquid chromatography, thin-layer chromatography and HPLC etc. However, these methods are time consuming. The separation and determination of stevia sweeteners by capillary electrophoresis was successfully demonstrated recently[1,2].

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