高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 339.

• Chemistry in Materials Sciences • 上一篇    下一篇

Instrument for Manipulating and Transporting Micro-particles in Liquid Phase

HU Yong-Gang1, HU Rui1, SHAO Han-Juan2, CHEN Mu-Zhuan2, TIAN Zhao-Wu3   

  1. 1. National Laboratory of Biomedical Photonics, Department of Environmental Science, Huazhong University of Science and Technology, Hubei 430074;
    2. The Key Laboratory of the Minitry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005;
    3 State Key Laboratory For Physical Chemistry of Solid Surface, Xiamen 361005
  • 出版日期:2000-12-31 发布日期:2000-12-31
  • 基金资助:

    Supported by the National Natural Science Foundation of China, Chenguang Project of Wuhan and Visiting Scholar Foundation of Key Lab. in University.

Instrument for Manipulating and Transporting Micro-particles in Liquid Phase

HU Yong-Gang1, HU Rui1, SHAO Han-Juan2, CHEN Mu-Zhuan2, TIAN Zhao-Wu3   

  1. 1. National Laboratory of Biomedical Photonics, Department of Environmental Science, Huazhong University of Science and Technology, Hubei 430074;
    2. The Key Laboratory of the Minitry of Education for Cell Biology and Tumor Cell Engineering, Xiamen University, Xiamen 361005;
    3 State Key Laboratory For Physical Chemistry of Solid Surface, Xiamen 361005
  • Online:2000-12-31 Published:2000-12-31
  • Supported by:

    Supported by the National Natural Science Foundation of China, Chenguang Project of Wuhan and Visiting Scholar Foundation of Key Lab. in University.

PDF (PC)

摘要：

More and more peoples begin to care for the ability to manipulate and transport single micro-particles such as cells, subcellular organelles and macromolecules for the purpose of study in biochemistry, biology, molecular medicine and molecular assemblies etc. According to the reports, the main methods for manipulating micro-particles in liquid phase are optical tweezers[1], electrophoresis[2] and suction syringe[3]. However, they are many shortcomings by using these methods mentioned above,for example, optical tweezers would lead to optical matter, electrophoresis method would lead to electric matter when the particles placed in the field, and the method of suction is difficult to reach automatization. Here, according to the theory of electroosmosis pump, a novel instrument so-called electro-micromanipulator developed in our laboratory for manipulating or transporting single micro-particles in liquid-phase is introduced. This electro-micromanipulator isolated the sample from electric field so that it could avoid the shortcomings of methods mentioned above. Finally, this instrument has been demonstrated for manipulate and transport different size single alga cells successfully,more research works of manipulating and transporting DNA macromolecules for study in biochemistry, biology, molecular assemblies etc are going on.

Abstract:

More and more peoples begin to care for the ability to manipulate and transport single micro-particles such as cells, subcellular organelles and macromolecules for the purpose of study in biochemistry, biology, molecular medicine and molecular assemblies etc. According to the reports, the main methods for manipulating micro-particles in liquid phase are optical tweezers[1], electrophoresis[2] and suction syringe[3]. However, they are many shortcomings by using these methods mentioned above,for example, optical tweezers would lead to optical matter, electrophoresis method would lead to electric matter when the particles placed in the field, and the method of suction is difficult to reach automatization. Here, according to the theory of electroosmosis pump, a novel instrument so-called electro-micromanipulator developed in our laboratory for manipulating or transporting single micro-particles in liquid-phase is introduced. This electro-micromanipulator isolated the sample from electric field so that it could avoid the shortcomings of methods mentioned above. Finally, this instrument has been demonstrated for manipulate and transport different size single alga cells successfully,more research works of manipulating and transporting DNA macromolecules for study in biochemistry, biology, molecular assemblies etc are going on.

TrendMD: