高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 17.

• Analytical Sciences • 上一篇    下一篇

BOD Biosensor Fabricated with Sol-Gel Membrane

CHEN Dan-Dan, CAO Yi-Bin, LIU Bao-Hong, LIU Zhen-Jiu, KONG Ji-Lie   

  1. Department of Chemistry, Fudan University, Shanghai 200433
  • 出版日期:2000-12-31 发布日期:2000-12-31
  • 基金资助:

    Project supported by Foundation for University Key Teacher by the Ministry of Education.

BOD Biosensor Fabricated with Sol-Gel Membrane

CHEN Dan-Dan, CAO Yi-Bin, LIU Bao-Hong, LIU Zhen-Jiu, KONG Ji-Lie   

  1. Department of Chemistry, Fudan University, Shanghai 200433
  • Online:2000-12-31 Published:2000-12-31
  • Supported by:

    Project supported by Foundation for University Key Teacher by the Ministry of Education.

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被引次数

7

摘要：

A novel BOD biosensor fabricated with the microbial membrane on an oxygen electrode was introduced by using porous A12O3 sol-gel inorganic matrix to immobilize the yeast, which is convenient, rapid, correlating to the results of BOD5 and could be employed for commercial availability in the future. The induced culture technique was used to obtain high active yeast, and this point especially contribute to the higher selectivity and sensitivity, The gelation time to form microorganisms membrane is about 1 h, and the response time is 20min, the response current is steady rapidly, a linear relationship between the response of the sensor and BOD value ranging from 10~70 mg/L, the reproducibility of BOD estimation is good and the lifetime of BOD biosensor is over 30 day at 4℃.

Abstract:

A novel BOD biosensor fabricated with the microbial membrane on an oxygen electrode was introduced by using porous A12O3 sol-gel inorganic matrix to immobilize the yeast, which is convenient, rapid, correlating to the results of BOD5 and could be employed for commercial availability in the future. The induced culture technique was used to obtain high active yeast, and this point especially contribute to the higher selectivity and sensitivity, The gelation time to form microorganisms membrane is about 1 h, and the response time is 20min, the response current is steady rapidly, a linear relationship between the response of the sensor and BOD value ranging from 10~70 mg/L, the reproducibility of BOD estimation is good and the lifetime of BOD biosensor is over 30 day at 4℃.

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