高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (S1): 152.

• Chemistry in Life Sciences • 上一篇    下一篇

The Effects of Citrato Molybdate on the Growth, Rate of Azotobacter Vinelandii

QIU Xue-Hui1, DONG En-Heng1, ZHOU Zhao-Hui2, LONG Ming-Nan1, ZHANG Feng-Zhang1, XU Liang-Shu1, WAN Hui-Lin2   

  1. 1. Department of Biology, Xiamen University, Xiamen 361005;
    2. Department of Chemistry, Xiamen University, Xiamen 361005
  • 出版日期:2000-12-31 发布日期:2000-12-31
  • 基金资助:

    Financial supports from the National Natural Science Foundation of China (Grant Nos. 39970176 and 29933040) are gratefully acknowledged.

The Effects of Citrato Molybdate on the Growth, Rate of Azotobacter Vinelandii

QIU Xue-Hui1, DONG En-Heng1, ZHOU Zhao-Hui2, LONG Ming-Nan1, ZHANG Feng-Zhang1, XU Liang-Shu1, WAN Hui-Lin2   

  1. 1. Department of Biology, Xiamen University, Xiamen 361005;
    2. Department of Chemistry, Xiamen University, Xiamen 361005
  • Online:2000-12-31 Published:2000-12-31
  • Supported by:

    Financial supports from the National Natural Science Foundation of China (Grant Nos. 39970176 and 29933040) are gratefully acknowledged.

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摘要：

An iron-molybdenum cofactor (FeMoco or M-cluster) of nitrogenase is believed to be the active center for the N2-binding and reduction. Recent X-ray crystal structure of MoFe-protein revealed the FeMoco as FeS3Fe3(S)3Fe3S3Mo(R-homocitrate) cluster and the biosynthesis of FeMoco requires at least six nif gene products. FeMoco should be assembled firstly and then inserted into the FeMoco-deficient dinitrogenase. It has been known that the NifB-co, the product of nifB, is an iron and sulfur-containing precursor of FeMoco. However, how the molybdenum and homocitrate are assembled into molybdenum free NifB-co remains unknown.

Abstract:

An iron-molybdenum cofactor (FeMoco or M-cluster) of nitrogenase is believed to be the active center for the N2-binding and reduction. Recent X-ray crystal structure of MoFe-protein revealed the FeMoco as FeS3Fe3(S)3Fe3S3Mo(R-homocitrate) cluster and the biosynthesis of FeMoco requires at least six nif gene products. FeMoco should be assembled firstly and then inserted into the FeMoco-deficient dinitrogenase. It has been known that the NifB-co, the product of nifB, is an iron and sulfur-containing precursor of FeMoco. However, how the molybdenum and homocitrate are assembled into molybdenum free NifB-co remains unknown.

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